Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity

Yi-Lin Gu, Jun-Zhou Li, Yan Li, Shen Cong, Jing Wang, Yi-Nan Ma, Hai-Lei Wei

Engineering ›› 2023, Vol. 28 ›› Issue (9) : 153-165.

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Engineering ›› 2023, Vol. 28 ›› Issue (9) : 153-165. DOI: 10.1016/j.eng.2023.05.016
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Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity

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Abstract

The multifunctional secondary metabolites known as cyclic lipopeptides (CLPs), which are produced by a large variety of bacteria, have become a key category of plant immunity elicitors. Pseudomonas-CLPs (Ps-CLPs) are extremely diverse in structure and biological activity. However, an understanding of CLP-plant structure-function interactions currently remains elusive. Here, we identify medpeptin, a novel CLP from Pseudomonas mediterranea that consists of 22 amino acids. Medpeptin is synthesized by a non-ribosomal peptide synthase (NRPS) gene cluster and regulated by a quorum-sensing system. Further research indicates that medpeptin does not exhibit antimicrobial activity; instead, it induces plant cell death immunity and confers resistance to bacterial infection. Comparative transcriptome analysis and virus-induced gene silencing (VIGS) reveal a set of immune signaling candidates involved in medpeptin perception. Silencing of a cell-wall leucine-rich repeat extensin protein (NbLRX3) or a receptor-like protein kinase (NbRLK25)—but not BAK1 or SGT1—compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana benthamiana. Our findings point to a noncanonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance.

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Pseudomonas / Cyclic lipopeptide / Cell death / Leucine-rich repeat extension (LRX) / Medpeptin / Receptor-like kinase (RLK)

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Yi-Lin Gu, Jun-Zhou Li, Yan Li, Shen Cong, Jing Wang, Yi-Nan Ma, Hai-Lei Wei. Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity. Engineering, 2023, 28(9): 153‒165 https://doi.org/10.1016/j.eng.2023.05.016

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