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role of cholesterol in modulation of morphology and mechanical properties of CHO-K1 cells: An in situ AFM

Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen

《化学科学与工程前沿（英文）》 2019年第13卷第1期页码 98-107 doi: 10.1007/s11705-018-1775-y

摘要：

Cholesterol plays a significant role in the organization of lipids and modulation of membrane dynamics in mammalian cells. However, the effect of cholesterol depletion on the eukaryotic cell membranes seems controversial. In this study, the effects of cholesterol on the topography and mechanical behaviors of CHO-K1 cells with manipulated membrane cholesterol contents were investigated by atomic force microscopy (AFM) technique. Here, we found that the depletion of cholesterol in cell membranes could increase the membrane stiffness, reduce the cell height as well as promote cell retraction and detachment from the surface, whereas the cholesterol restoration could reverse the effect of cholesterol depletion on the membrane stiffness. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. This research demonstratede importance of cholesterol in regulating the dynamics of cytoskeleton-mediated processes. AFM technique offers excellent advantages in the dynamic monitoring of the change in membranes mechanical properties and behaviors during the imaging process. This promising technology can be utilized in studying the membrane properties and elucidating the underlying mechanism of distinct cells in the near-native environment.

关键词： cholesterol methyl-β-cyclodextrin atomic force microscopy Young’s modulus CHO-K1 cell

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基于AFM探针的纳米加工技术制备周期性纳米结构——沟槽和材料堆积形貌相结合 Article

耿延泉, 闫永达, 王继强, Emmanuel Brousseau, 孙彦文, 孙雅洲

《工程（英文）》 2018年第4卷第6期页码 787-795 doi: 10.1016/j.eng.2018.09.010

摘要：

本文提出了一种基于原子力显微镜（AFM）探针的加工技术制备周期性纳米结构的方法，该方法通过将加工得到的沟槽形貌和沟槽边缘的材料堆积形貌相结合得到周期性纳米结构。在检测得到周期结构的AFM 图像中，取其截面进行FFT并将幅频特性曲线归一化，在幅频特性曲线中用峰值部分面积与总体面积的比值来定量分析纳米结构的周期性。

关键词：原子力显微镜纳米加工周期性纳米结构单晶铜

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Simulation of abrasive flow machining process for 2D and 3D mixture models

Rupalika DASH,Kalipada MAITY

《机械工程前沿（英文）》 2015年第10卷第4期页码 424-432 doi: 10.1007/s11465-015-0366-6

摘要：

Improvement of surface finish and material removal has been quite a challenge in a finishing operation such as abrasive flow machining (AFM). Factors that affect the surface finish and material removal are media viscosity, extrusion pressure, piston velocity, and particle size in abrasive flow machining process. Performing experiments for all the parameters and accurately obtaining an optimized parameter in a short time are difficult to accomplish because the operation requires a precise finish. Computational fluid dynamics (CFD) simulation was employed to accurately determine optimum parameters. In the current work, a 2D model was designed, and the flow analysis, force calculation, and material removal prediction were performed and compared with the available experimental data. Another 3D model for a swaging die finishing using AFM was simulated at different viscosities of the media to study the effects on the controlling parameters. A CFD simulation was performed by using commercially available ANSYS FLUENT. Two phases were considered for the flow analysis, and multiphase mixture model was taken into account. The fluid was considered to be a Newtonian fluid and the flow laminar with no wall slip.

关键词： abrasive flow machining (AFM) computational fluid dynamics (CFD) modeling mixture model

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The investigation of fly ash based asphalt binders using atomic force microscope

Rajan SAHA, Kyle MALLOY, Emil BAUTISTA, Konstantin SOBOLEV

《结构与土木工程前沿（英文）》 2017年第11卷第4期页码 380-387 doi: 10.1007/s11709-017-0437-3

摘要： Atomic Force Microscope (AFM) is a relatively new technique for investigation of construction materials. In this study AFM was used to investigate the interaction of asphalt binder with fly ash. Fly ash is a coal combustion byproduct of electric power utilities having pozzolanic properties and commonly used in Portland cement concrete. In this study, an investigation was made by using different types of fly ash with two types of asphalt binders such as PG 58-28 and PG 64-28. Asphalt microstructure is divided into four subgroups such as Saturates, Aromatics, Resins and Asphaltenes (SARA). These four phases can be distinguished by the atomic force microscope. The interaction of these phases affected by introducing fly-ash was investigated and correlation with rheological properties was observed.

关键词： AFM fly ash bee rheology asphalt

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基于磁驱动正交悬臂探针的三维原子力显微镜技术开发 Article

张号, 耿俊媛, 高海波, 荣伟彬, 谢晖

《工程（英文）》 2023年第24卷第5期页码 84-93 doi: 10.1016/j.eng.2022.06.010

摘要： driven-orthogonal cantilever probes, MD-OCP）的三维原子力显微镜（three-dimensional atomic force microscopy, 3D-AFM该3D-AFM系统还配备了高精度旋转台，可实现360°全向成像。定制的MD-OCP包含水平悬臂、垂直悬臂和磁珠三部分，其中磁珠可在磁场中机械驱动OCP实现激振。接着，使用标准AFM阶梯光栅进行对比实验，并结合三维形貌重建方法完成了图像整合，验证了所提出方法面向陡峭侧壁和拐角处细节的表征能力。通过对具有微梳结构的微机电系统（MEMS）器件进行3D表征，进一步证实了所提出的基于MD-OCP的3D-AFM技术的有效性。

关键词：三维原子力显微镜磁驱动正交悬臂探针关键尺寸 360°表征三维形貌重建