Frontiers of Information Technology & Electronic Engineering
>> 2021,
Volume 22,
Issue 10
doi:
10.1631/FITEE.2000410
Enhanced collection of scattered photons in nonlinear fluorescence microscopy by extended epi-detection with a silicon photomultiplier array
Affiliation(s): State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China; IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China; less
Received: 2020-08-12
Accepted: 2021-10-08
Available online: 2021-10-08
Next
Previous
Abstract
To maximize signal collection in nonlinear optical microscopy, non-descanned epi-detection is generally adopted for in vivo imaging. However, because of severe scattering in biological samples, most of the emitted fluorescence photons go beyond the collection angles of objectives and thus cannot be detected. Here, we propose an extended detection scheme to enhance the collection of scattered photons in using a ahead of the front apertures of objectives. We perform numerical simulations to demonstrate the enhanced fluorescence collection via in the multi-photon fluorescence imaging of human skin and mouse brain through craniotomy windows and intact skulls. For example, with red fluorescence emission at a depth of 600 μm in human skin, the increased collection can be as much as about 150% with a 10×, 0.6-NA objective. We show that is a generally applicable, feasible technique for use in to enhance signal detection.
京公网安备 11010502051620号
