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High End Computing Software Platform R & D
Chen Zuoning
Strategic Study of CAE 2002, Volume 4, Issue 9, Pages 45-49
High end computing software platform is the basic guarantee for implementing the friendship, availability and high efficiency of the entire machine. With the ever increase of computing scale and the emerging of new computing patterns, such as Peer-to-peer and Grid, the design of software platform faces new challenges. This paper describes and analyses the development of high end computing software platform and the designing difficulties, which it faces. Then the design case of a certain high end computing platform software system will be introduced and discussed here.
Keywords： high end computing MPP OS parallel development environment parallel application environment
Povedano, Maria Gamella, Rebeca Magnolia Torrente-Rodríguez, Miren Alonso-Navarro, Vivian de los Ríos
Engineering 2021, Volume 7, Issue 10, Pages 1393-1412 doi: 10.1016/j.eng.2021.04.026
Colorectal cancer (CRC) is the second leading cause of cancer related death worldwide. The 5-year survival rate of CRC patients depends on the stage at diagnosis, being higher than 80% when CRC is diagnosed in the early stages but lower than 10% when CRC is diagnosed in advanced stages. Autoantibodies against specific CRC autoantigens (tumor-associated antigens (TAAs)) in the sera of patients have been widely demonstrated to aid in early diagnosis. Thus, we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy. To that end, we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis. A total of 103 proteins were identified as potential autoantigens specific to CRC. After bioinformatics and meta-analysis, we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot (WB) and immunohistochemistry (IHC). We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients, along with an association of nine of these proteins with CRC prognosis. After validation, all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals, either by luminescence Halotag-based beads, or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection. Taken together, our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases; they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care (POC) device for CRC detection with high diagnostic ability.
Keywords： Autoantibodies Diagnosis Colorectal cancer Exosomes Tumor microenviroment Humoral immune response Point of care Biosensors
Title Author Date Type Operation
The Harmony operating system (OS)
20 Dec 2023
Conference Videos
Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer
Povedano, Maria Gamella, Rebeca Magnolia Torrente-Rodríguez, Miren Alonso-Navarro, Vivian de los Ríos
Journal Article
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