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Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG
Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0844-z
Keywords： Preozonation BAC filtration High ammonia level Bacterial activity Microbial community structure Pilot scale study
Development and clinical trial of a novel bioactive bone cement
LEONG John, LI Zhaoyang, LU William
Frontiers of Medicine 2008, Volume 2, Issue 2, Pages 117-126 doi: 10.1007/s11684-008-0022-1
Keywords： bioactivity effectiveness strontium-containing hydroxyapatite osseointegration sufficient mechanical
Xu Yongjiang,Zang Kun,Liu Xuezhou,Shi Bao and Chen Shengyi
Strategic Study of CAE 2015, Volume 17, Issue 1, Pages 67-73
The mature peptide domain of insulin-like growth factor-I from Platichthys stellatus were amplified with specific primers based on its cDNA sequence. Then the matured peptide fragment was subcloned into the prokaryotic expression vector pET-28a to successfully construct IGF-I/pET-28a recombinant plasmid which were highly expressed in E.coli BL21（DE3） after being induced by IPTG with special fusion polypeptides containing His6 at their N-terminus. The obtained IGF-I polypeptide expressed in form of inclusion bodies with molecular weight of 12.1 kD and maximally accounted for 39.8 % of the whole bacterial protein post 3 h induction with 0.5 mmol/L IPTG at 37 ℃. Western blotting analysis indicated fusion polypeptides had the antigenicity to His6 antibody. The inclusion bodies were denaturalized using 6 mol/L guanidine HCl， purified using Ni-NTA affinity chromatography and annealed by gradient dialysis in urea， then purified proteins with molecular weight of 12.1 kD which was obtained from IGF-I recombinant bacterium. The proliferation experiment showed recombinant IGF-I protein could significantly promote the proliferation of human embryonic kidney cells HEK293T at 0.6 μg/mL and inhibit the proliferation with 1.8 μg/mL which verified its biological activity. Therefore， the IGF-I prokaryotic expression system was successfully constructed in the present study and biologically active IGF-I fusion protein was obtained. The present results would be helpful for better understanding the roles of IGF-I in growth regulation and development of high effective additive for growth promotion of Platichthys stellatus.
Keywords： Platichthys stellatus； insulin-like growth factor-I； prokaryotic expression； bioactivity
High-expression of recombinant human concensus interferon-α by Pichia pastoris
HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin
Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4, Pages 399-403 doi: 10.1007/s11705-007-0073-x
Keywords： bioactivity pastoris maximum induction optimized
Title Author Date Type Operation
Impact of preozonation on the bioactivity and biodiversity of subsequent biofilters under low temperature
Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG
Journal Article
Development and clinical trial of a novel bioactive bone cement
LEONG John, LI Zhaoyang, LU William
Journal Article
Prokaryotic expression and bioactivity analysis of growth hormone and insulin-like growth factor-I from
Xu Yongjiang,Zang Kun,Liu Xuezhou,Shi Bao and Chen Shengyi
Journal Article
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