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Journal Article 2

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2015 1

2014 1

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CRISPR/Cas9 1

human lactoferrin bacterial artificial chromosome (hLF BAC) 1

lactoferrin 1

milk 1

plasmid pX330 1

promoter 1

recombinant human butyrylcholinesterase (rhBCHE) 1

transgenic mice 1

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: Lactoferrin is a member of the transferrin family of multifunctional iron binding glycoproteins.While numerous physiological functions have been described for lactoferrin, the mechanisms underlyingTo further study the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter ofmice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.Seven mice with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injecting

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: In this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF    

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Title Author Date Type Operation

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article