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denaturing gradient gel electrophoresis (DGGE) 3

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Relative writing age analysis of the black roller pen inksby capillary zone electrophoresis

Wang Yanji,Zhao Pengcheng,Xu Yuanyuan, Wan Jinghan

Strategic Study of CAE 2007, Volume 9, Issue 2,   Pages 25-29

Abstract: text-align: justify;"> The analytical method for roller pen inks was established by capillary zone electrophoresis.After

Keywords： black roller pen inks     zone capillary electrophoresis     writin    

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Application of proteomics in environmental science

Xiaona CHU, Jiangyong HU, Say Leong ONG,

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 4,   Pages 393-403 doi: 10.1007/s11783-009-0145-x

Abstract: Proteomics involves the separation of proteins, identification of the amino acid sequence of the interested or target proteins, study of the function of the proteins, modification, structure and ultimate assignments to functional pathways in the cell. The proteomic investigations have contributed greatly to human diseases studies, new drugs discovery researches, and environmental science in recent years. This article provides a review on the development of the main proteomic technologies, including both the gel based and non-gel based technologies, and their applications in environmental science. Proteomic technologies have been utilized in the environmental stresses studies to analyze the induction or reduction of proteins at expression level and identify the target proteins to investigate their function in response to environmental stresses, such as high or low pH, oxidation stress, and toxic chemicals. Such protein responses are also helpful to understand the mechanisms of some cellular activities and the functions of some proteins.

Keywords： proteomics     environmental stress     two dimensional (2D) gel electrophoresis     mass spectrometry    

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Genetics analysis of haptoglobin gene in Fujian Han nationality

ZOU Qilian, LIN Ying, LIN Xiangquan, CHEN Yuanzhong

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 105-107 doi: 10.1007/s11684-008-0019-9

Abstract: The phenotypes of the haptoglobin of four different groups were analyzed by using polyacrylamide gel electrophoresis

Keywords： different     haptoglobin     phenotype frequency     electrophoresis     middle    

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Purification and characterization of a novel carbonyl reductase with high stereo-selectivity

YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 404-410 doi: 10.1007/s11705-007-0074-9

Abstract: The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Keywords： asymmetric synthesis     sulfate-polyacrylamide     purified     electrophoresis     temperature    

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Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 82-97 doi: 10.1007/s11783-011-0306-6

Abstract: Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

Keywords： molecular biological technique     microbial community     denaturing gradient gel electrophoresis (DGGE)     terminal    

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Investigation of electrochemical degradation and application of e-paper dyes in organic solvents

Luhai LI, Ming WANG, Yi FANG, Shunan QIAO

Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 2,   Pages 182-185 doi: 10.1007/s11705-009-0062-3

Abstract: To avoid environmental pollution due to organic dye solutions, the electrophoresis and degradation ofBoth the curves of ultraviolet-visible (UV-Vis) and infrared (IR) spectra of the electrophoresis samplesA dye electrophoresis in organic solution flexible prototype e-paper display was formed and the display

Keywords： electrochemical degradation     electrophoresis     e-paper     oxidized azobenzene group    

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Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 40-47 doi: 10.1007/s11783-011-0304-8

Abstract: analyze the microbial community shift along with environmental variations using denaturing gradient gel electrophoresis

Keywords： sewage treatment plants     polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE    

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Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords： quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

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Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 196-205 doi: 10.1007/s11684-017-0560-5

Abstract:

We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE

Keywords： Clostridium difficile     multiplex PCR     capillary electrophoresis     detection     characterization    

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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Engineering doi: 10.1016/j.eng.2023.02.016

Abstract: Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis    

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PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China

Wei Li, Shan Lu, Zhigang Cui, Jinghua Cui, Haijian Zhou, Yiqing Wang, Zhujun Shao, Changyun Ye, Biao Kan, Jianguo Xu

Frontiers of Medicine 2012, Volume 6, Issue 4,   Pages 366-375 doi: 10.1007/s11684-012-0214-6

Abstract:

Surveillance is critical for the prevention and control of infectious disease. China’s real-time web-based infectious disease reporting system is a distinguished achievement. However, many aspects of the current China Infectious Disease Surveillance System do not yet meet the demand for timely outbreak detection and identification of emerging infectious disease. PulseNet, the national molecular typing network for foodborne disease surveillance was first established by the Centers for Disease Control and Prevention of the United States in 1995 and has proven valuable in the early detection of outbreaks and tracing the pathogen source. Since 2001, the China CDC laboratory for bacterial pathogen analysis has been a member of the PulseNet International family; and has been adapting the idea and methodology of PulseNet to develop a model for a future national laboratory-based surveillance system for all bacterial infectious disease. We summarized the development progress for the PulseNet China system and discussed it as a model for the future of China’s national laboratory-based surveillance system.

Keywords： infectious disease     laboratory-based infectious disease surveillance     pulse field gel electrophoresis     multilocus    

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Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 2,   Pages 324-333 doi: 10.1007/s11783-014-0627-3

Abstract: community structure of the granules was also analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis

Keywords： continuous flow     shear force     filamentous bacteria     polymerase chain reaction-denaturing gradient gel electrophoresis    

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Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Engineering 2023, Volume 26, Issue 7,   Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

Abstract: mdash;to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis    

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Title Author Date Type Operation

Relative writing age analysis of the black roller pen inksby capillary zone electrophoresis

Wang Yanji,Zhao Pengcheng,Xu Yuanyuan, Wan Jinghan

Journal Article

Application of proteomics in environmental science

Xiaona CHU, Jiangyong HU, Say Leong ONG,

Journal Article

Genetics analysis of haptoglobin gene in Fujian Han nationality

ZOU Qilian, LIN Ying, LIN Xiangquan, CHEN Yuanzhong

Journal Article

Purification and characterization of a novel carbonyl reductase with high stereo-selectivity

YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong

Journal Article

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Journal Article

Investigation of electrochemical degradation and application of e-paper dyes in organic solvents

Luhai LI, Ming WANG, Yi FANG, Shunan QIAO

Journal Article

Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article

Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Journal Article

Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Journal Article

PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China

Wei Li, Shan Lu, Zhigang Cui, Jinghua Cui, Haijian Zhou, Yiqing Wang, Zhujun Shao, Changyun Ye, Biao Kan, Jianguo Xu

Journal Article

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Journal Article

Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Journal Article