Resource Type

Journal Article 6

Year

2017 1

2009 2

2008 1

2007 2

Keywords

control 2

β 1

-catenin 1

E-cadherin 1

PP2A activity 1

RNAi therapeutics 1

Survivin-1 1

actin 1

apoptosis 1

cell cycle 1

cellular regulation 1

colorectal carcinoma 1

cyclin-dependent kinase1 1

cytoplasm 1

dendrimer 1

gene silencing 1

gene therapy 1

growth 1

hyperphosphorylation 1

hypoxia 1

open ︾

Search scope:

排序： Display mode:

Influence of Survivin-targeted siRNA on the biological features of colorectal carcinoma cells

XIONG Ying, GUO Wen, LI Ting, LI Ke

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 304-307 doi: 10.1007/s11684-007-0058-7

Abstract: The transient transfection of survivin-targeted siRNA to Lovo cells and its influence on the biologicalTwo pairs of 19 base pairs (bp) siRNA-specific targeted survivin gene were designed and synthesized by

Keywords： control     therapy     influence     Survivin-1     colorectal carcinoma    

HTML PDF Collect

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 663-675 doi: 10.1007/s11705-017-1623-5

Abstract: Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as longDendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecturebeen established, some of them out-performing the currently available nonviral vectors in delivering siRNAadvantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNAself-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA

Keywords： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

HTML PDF Collect

The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 384-389 doi: 10.1007/s11684-009-0070-1

Abstract: In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence theThe siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamineCDK1 gene was successfully silenced by its siRNA, and the CDK1 protein expression level was decreasedThe DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G/M phaseThe decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G/M phase but did

Keywords： cyclin-dependent kinase1     siRNA interference     cell cycle     apoptosis    

HTML PDF Collect

Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 303-308 doi: 10.1007/s11684-009-0060-3

Abstract: The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H (non-transfected Hela cells), H (pGenesil-1 empty plasmid-transfected Hela cells), and H (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O) and normoxia for 48h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch test and the invasion assay were applied to examine the invasion in each group. The expression of HIF-1α, E-cadherin, and β-catenin in tumor grafts was assayed immunohistochemically. Western blot results revealed that the bands of HIF-1α, E-cadherin, β-catenin, and actin proteins were detected in the H and H groups under hypoxia for 48h. The expression of E-cadherin, β-catenin, and actin was detected in the H group under hypoxia for 48h, and normoxia. In the H, H, and H groups under normoxia, and the H group under hypoxia for 48h, no expression of HIF-1α was detectable. The scratch test showed that the invasive ability in the H group was significantly alleviated. Immunohistochemically, it was found that there was a significant difference in the expression of HIF-1α, E-cadherin, and β-catenin between the H and H groups (<0.05), but the difference was not significant between the H and H groups. It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins (E-cadherin, β-catenin, and actin) depend on HIF-1α.

Keywords： hypoxia     actin     β     -catenin     E-cadherin     invasion    

HTML PDF Collect

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 235-238 doi: 10.1007/s11684-008-0044-8

Abstract: Specific Src siRNA was transfected into cultured mouse neuroblastoma N2a cells to inhibit the expressionY307 and tau at different sites, as well as PP2A activity were detected at different time points after siRNATwelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreased

Keywords： hyperphosphorylation     PP2A activity     cellular regulation     siRNA     siRNA transfection    

HTML PDF Collect

Impact of siRNA targeting pirh2 on proliferation and cell cycle control of the lung adenocarcinoma cell

SU Yuan, JIN Yang, ZHANG Xiaoju, ZHOU Qiong, BAI Ming, ZHU Liping

Frontiers of Medicine 2007, Volume 1, Issue 4,   Pages 359-363 doi: 10.1007/s11684-007-0069-4

Abstract: The inhibition of pirh2 expression by siRNA (psiRNA-pirh2) resulted in reduced cell proliferation and

Keywords： control     interruption     cytoplasm     number     growth    

HTML PDF Collect

Title Author Date Type Operation

Influence of Survivin-targeted siRNA on the biological features of colorectal carcinoma cells

XIONG Ying, GUO Wen, LI Ting, LI Ke

Journal Article

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Journal Article

The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

Journal Article

Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

Journal Article

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Journal Article

Impact of siRNA targeting pirh2 on proliferation and cell cycle control of the lung adenocarcinoma cell

SU Yuan, JIN Yang, ZHANG Xiaoju, ZHOU Qiong, BAI Ming, ZHU Liping

Journal Article