A simple umbelliferone based fluorescent probe for the detection of nitroreductase

2018年第12卷第2期

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《化学科学与工程前沿（英文）》 >> 2018年第12卷第2期 doi: 10.1007/s11705-017-1697-0

A simple umbelliferone based fluorescent probe for the detection of nitroreductase

. Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

录用日期： 2018-01-15 发布日期： 2018-05-09

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摘要

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

关键词

fragmentation ; NCOU1 ; NTR ; nitroreductase ; nitrobenzyl-umbelliferone