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Advantages of intermittently aerated SBR over conventional SBR on nitrogen removal for the treatment of digested

Xiaoyan Song, Rui Liu, Lujun Chen, Baogang Dong, Tomoki Kawagishi

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0941-7

摘要： An intermittently aerated sequencing batch reactor (IASBR) and a traditional sequencing batch reactor (SBR) were parallelly constructed to treat digested piggery wastewater, which was in high NH -N concentration but in a low COD/TN ratio. Their pollutant removal performance was compared under COD/TN ratios of 1.6–3.4 d and hydraulic retention times of 5–3 d. The results showed that the IASBR removed TN, NH -N and TOC more efficiently than the SBR. The average removal rates of TN, NH -N and TOC were 83.1%, 96.5%, and 89.0%, respectively, in the IASBR, significantly higher than the corresponding values of 74.8%, 82.0%, and 86.2% in the SBR. Mass balance of organic carbon revealed that the higher TN removal in the IASBR might be attributed to its efficient utilization of the organic carbon for denitrification, since that 48.7%–52.2% of COD was used for denitrification in the IASBR, higher than the corresponding proportion of 43.1%–47.4% in the SBR. A pre-anoxic process in the IASBR would enhance the ammonium oxidation while restrict the nitrite oxidation. Anoxic duration of 40–80 min should be beneficial for achieving stable nitritation.

关键词： Anoxic stress     Carbon source     Digested piggery wastewater     Intermittently aerated SBR (IASBR)     Total nitrogen    

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Comparative experiment on treating digested piggery wastewater with a biofilm MBR and conventional MBR

Xiaoyan Song,Rui Liu,Lujun Chen,Tomoki Kawagishi

《环境科学与工程前沿（英文）》 2017年 第11卷 第2期 doi: 10.1007/s11783-017-0919-5

摘要： The BF-MBR displayed higher removal rates of nitrogen, phosphorous and antibiotics. The BF-MBR saved alkali consumption. The removal of antibiotics was influenced significantly by HRT. Membrane filtration greatly contributed to antibiotics removal. A biofilm membrane bioreactor (BF-MBR) and a conventional membrane bioreactor (MBR) were parallelly operated for treating digested piggery wastewater. The removal performance of COD, TN, NH -N, TP as well as antibiotics were simultaneously studied when the hydraulic retention time (HRT) was gradually shortened from 9 d to 1 d and when the ratio of influent COD to TN was changed. The results showed that the effluent quality in both reactors was poor and unstable at an influent COD/TN ratio of 1.0±0.2. The effluent quality was significantly improved as the influent COD/TN ratio was increased to 2.3±0.5. The averaged removal rates of COD, NH -N, TN and TP were 92.1%, 97.1%, 35.6% and 54.2%, respectively, in the BF-MBR, significantly higher than the corresponding values of 91.7%, 90.9%, 17.4% and 31.9% in the MBR. Analysis of 11 typical veterinary antibiotics (from the tetracycline, sulfonamide, quinolone, and macrolide families) revealed that the BF-MBR removed more antibiotics than the MBR. Although the antibiotics removal decreased with a shortened HRT, high antibiotics removals of 86.8%, 80.2% and 45.3% were observed in the BF-MBR at HRT of 5–4 d, 3–2 d and 1 d, respectively, while the corresponding values were only 83.8%, 57.0% and 25.5% in the MBR. Moreover, the BF-MBR showed a 15% higher retention rate of antibiotics and consumed 40% less alkalinity than the MBR. Results above suggest that the BF-MBR was more suitable for digested piggery wastewater treatment.

关键词： Alkalinity     Antibiotics     Biofilm     Digested piggery wastewater (DPW)     Membrane bioreactor    

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community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0929-3

摘要： A traditional sequencing batch reactor (SBR) and two intermittently aerated sequencing batch reactors (IASBRs) were parallelly operated for treating digested piggery wastewater. Their microbial communities were analyzed, and the nitrogen removal performance was compared during the long–term run. IASBRs demonstrated higher removal rates of total nitrogen (TN) and ammonium nitrogen (NH -N) than the SBR, and also demonstrated higher resistance against TN shock load. It was found that the more switch times between aerobic/anoxic in an IASBR, the higher the removal rates of TN and NH –N. All the reactors were predominated by , and , which were considered to be species of denitrifiers, ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. However, the abundance and diversity was of great difference. Compared with SBR, IASBRs achieved higher abundance of denitrification–related bacteria. IASBR 1 with four aerobic/anoxic switch times was detected with 25.63% of , higher than that in IASBR 2 with two aerobic/anoxic switch times (11.57% of ), and much higher than that in the SBR (only 6.19% of ). IASBR 2 had the highest percentage of AOB, while IASBR 1 had the lowest percentage. The denitrifiers abundance was significantly positive correlated with the TN removal rate. However, the NH –N removal rate showed no significant correlation with the AOB abundance, but might relate to the AOB activity which was influenced by the average free ammonium (FA) concentration. was the only NOB genus detectable in all reactors, and were less than 0.03%.

关键词： Digested piggery wastewater     Intermittent aeration     Microbial community     Partial nitrification–denitrification process     Sequencing batch reactor (SBR)    

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removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested

Zhenfeng Han, Ying Miao, Jing Dong, Zhiqiang Shen, Yuexi Zhou, Shan Liu, Chunping Yang

《环境科学与工程前沿（英文）》 2019年 第13卷 第4期 doi: 10.1007/s11783-019-1133-4

摘要：

Anaerobically digested swine wastewater was treated by a novel constructed wetland.

Tidal operation was better for total nitrogen removal than intermittent flow.

Mechanism of nitrogen removal by biozeolite-based constructed wetland was discussed.

Simultaneous nitrification and denitrification were determined in zeolite layer.

关键词： Constructed wetland     Decentralized swine wastewater     Biozeolite     Simultaneous nitrification and denitrification     Nitrogen removal pathway    

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Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

《医学前沿（英文）》 2008年 第2卷 第1期   页码 35-38 doi: 10.1007/s11684-008-0007-0

摘要： The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection on CD2AP distribution in HEK293 cells. The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned and digested with HI and I. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plasmids were transfected into HEK293 cells and its effects on CD2AP distribution were observed by immunofluorescence. The pEGFP-NPHS2 expression vector was successfully constructed and podocin exclusively located on the HEK293 cell membrane. After podocin transfection, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells. It can be concluded that podocin can recruit CD2AP to redistribute from the perinucleus to the cytoplasm in HEK293 cells.

关键词： pEGFP-NPHS2 expression     transfection     pEGFP-C2     cytoplasm     digested    

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标题 作者 时间 类型 操作

Advantages of intermittently aerated SBR over conventional SBR on nitrogen removal for the treatment of digested

Xiaoyan Song, Rui Liu, Lujun Chen, Baogang Dong, Tomoki Kawagishi

期刊论文

Comparative experiment on treating digested piggery wastewater with a biofilm MBR and conventional MBR

Xiaoyan Song,Rui Liu,Lujun Chen,Tomoki Kawagishi

期刊论文

community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

期刊论文

removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested

Zhenfeng Han, Ying Miao, Jing Dong, Zhiqiang Shen, Yuexi Zhou, Shan Liu, Chunping Yang

期刊论文

Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

期刊论文