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2008 1

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Improvement of detection method of and in reclaimed water

ZHANG Tong, XIE Xing, HU Hongying, SONG Yudong, WU Qianyuan, ZONG Zusheng

《环境科学与工程前沿（英文）》 2008年 第2卷 第3期   页码 380-384 doi: 10.1007/s11783-008-0052-6

摘要： and are two typical species of pathogenic protozoans that seriously endanger water quality. Previous works indicated that detection of and with modified United States Environmental Protection Agency (USEPA) method-1623 using a membrane filtration-elution for sample concentration attained better recovery and lower cost compared to the USEPA method-1623. Several improvements of membrane filtration-elution step as well as immunomagnetic separation (IMS) steps were investigated and an optimized method for detection of and in wastewater reclamation system was recommended in this paper. The experimental results show that an overnight soak of the membrane after scraping and vortex agitation before elution could enhance and stabilize the recovery. Increasing turbidity to 4 NTU by adding kaolin clay before filtration could effectively improve the recovery of low-turbidity water. Washing the concentrate after centrifugation and twice acid dissociation both reduced the impact of water quality to protozoan recovery. Protozoans in different water samples were determined by this optimized method, and the recovery of and were above 70% and 80% respectively, much higher than the acceptance of method-1623.

关键词： USEPA method-1623     Environmental Protection     wastewater reclamation     concentration     centrifugation    

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Improvement of detection method of and in reclaimed water

ZHANG Tong, XIE Xing, HU Hongying, SONG Yudong, WU Qianyuan, ZONG Zusheng

期刊论文