Frontiers of Agricultural Science and Engineering
>> 2014,
Volume 1,
Issue 3
doi:
10.15302/J-FASE-2014024
RESEARCH ARTICLE
Transcriptome analysis of wheat grain using RNA-Seq
1. Beijing Key Laboratory of Crop Genetic Improvement,College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.2. Key Laboratory of Crop Heterosis & Utilization, Ministry of Education,College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Accepted: 2014-12-01
Available online: 2015-01-27
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Abstract
With the increase in consumer demand, wheat grain quality improvement has become a focus in China and worldwide. Transcriptome analysis is a powerful approach to research grain traits and elucidate their genetic regulation. In this study, two cDNA libraries from the developing grain and leaf-stem components of bread wheat cultivar, Nongda211, were sequenced using Roche/454 technology. There were 1061274 and 1516564 clean reads generated from grain and leaf-stem, respectively. A total of 61393 high-quality unigenes were obtained with an average length of 1456 bp after assembly. The analysis of the 61393 unigenes involved in the biological processes of the grain showed that there were 7355 differentially expressed genes upregulated in the grain library. Gene ontology enrichment and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that many transcription products and transcription factors associated with carbohydrate and protein metabolism were abundantly expressed in the grain. These results contribute to excavate genes associated with wheat quality and further study how they interact.
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