Frontiers of Agricultural Science and Engineering
>> 2015,
Volume 2,
Issue 1
doi:
10.15302/J-FASE-2015048
RESEARCH ARTICLE
Novel polymorphic EST-based microsatellite marker isolation and characterization from
1. Key Laboratory of Horticultural Plant Biology (MOE), National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China.2. Desert Research Center, Egyptian Deserts Gene Bank, North Sinai, Egypt
Accepted: 2015-05-05
Available online: 2015-05-22
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Abstract
Novel simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.