采用密度梯度离心法分离骨髓间质干细胞，差速贴壁法进行纯化，应用流式细胞仪、激光共聚焦显微镜等对纯化细胞进行鉴定，结果细胞表面抗原CD29，CD44，CD105，CD166表达呈阳性，而CD14，CD34，CD45表达呈阴性。采用RT唱PCR鉴定了三个基因：nestin，NST，Oct-４，前两者呈阳性表达，后者弱阳性表达。这些细胞特异抗原与基因表达综合起来，表明得到的细胞具备骨髓间质干细胞的特性。密度梯度分离与差速贴壁相结合，可获得较好均一性的骨髓间质干细胞，是一种简单可靠、易于推广的骨髓干细胞获取方法，可为细胞与组织工程研究提供种子细胞。

Bone marrow mesenchymal stem cells (BMMSCs) were separated by density gradient centrifugation and purified by different adherence speed. BMMSC was identified by flow cytometry analysis and laser confocal microscopy. Cell surface antigen (CSA) CD29, CD44, CD105, CD166 were detected positive expression and CD14, CD34, CD45 were not positive. The positive expression of the nestin, NST, and weakly positive expression of Oct-4 were confirmed by RT-PCR. The cells were verified as the BMMSC by the expression of the CSA and the genes. Using density gradient separation and different adherence speed could harvest high quality BMMSC. This is a simple and reliable method to obtain BMMSC as stem cell used in cell and tissue engineering research.