目的：项目拟通过观察在培养液中加入较高剂量的人参皂苷CK（Ginsenoside，G组）、淫羊藿苷（Icraiin，I组）两种药物前﹑后精子死亡率数据，评估两种药物单体在较大剂量时对体外精子的死亡率的影响。方法：新鲜液化的精液（semen）标本40份，F-10培养液调整浓度为1.0×107/mL，分别添加不同剂量的人参皂苷CK（Ginsenoside，Gin CK）﹑淫羊藿苷（Icraiin，Icr）按照不同药物浓度，G组分为500 μg/mL组（G1组），100 μg/mL组（G2组），I组分为500 μg/mL（I1组），100 μg/mL（I2组）；设不添加任何物质的F-10组（F-10，F组）为阴性对照组，培养24 h后分别检查各组精子活动率和死亡率。结果：培养24 h后，精子死亡率从低到高分别是培养24 h后，I2组死亡率为41.31±17.49， F组死亡率为47.50±19.85 ，G2组死亡率为55.75±24.93，I3组死亡率为 58.05±19.06，G1组死亡率为98.84±1.62。精子死亡率从低到高分别是I2组﹑F组﹑G2组﹑I1组﹑G1组，各组比较有差异（P<0.05）。结论：较大剂量的Gin CK、Icr，均可引起体外培养的精子死亡率增高；在0.1 mg/mL的浓度下的Gin CK对精子的致死作用已经比较明显，而0.5 mg/mL对体外培养精子来说是致死剂量（98.84 %），在0.1 mg/mL的剂量下的淫羊藿苷对精子的致死作用并不明显，在0.5 mg/mL剂量下Icr对精子致死作用有所提高，但远未达到Gin CK 98.4%的致死率。

Objective：To investigate the effects of Ginsenoside CK(Gin CK),Icraiin(Icr) for sperm survive in vitro. Methods：40 sample of male semen were cultured in medium and grouped it in vitro,To adjust the semen concentration is 1.0×107/mL by culture medium of F-10(F-10),To add respect Ginsenoside(Gin1 group,500 μg/mL；Gin2 group,100 μg/mL),Icraiin(Icr1 group,500 μg/mL；Icr2 group,100 μg/mL)were added respectively. F-10 alone was the control groups. To compare with them of groups sperm tmortality. Results：The values of sperm mortality after cultured for 24 h. The motality of I2 group was 41.31±17.49,F group was 47.50±19.85,G2 group was 55.75±24.93,I3 group was 58.05±19.06,G1 group was 98.84±1.62. Ranking them from less to more motality were E2 groups,F groups,I2 groups,G2 groups,I3 groups,G1 group s(p<0.05). Conclusion：When the concentration was 0.1 mg/mL,Gin CK can increase the sperm mortality significantly and Icr was not; When the concentration was 0.5 mg/mL,it is the lethal dose for Gin CK and Icr can increase the sperm mortality but it is not.