Objective：To investigate the effects of Ginsenoside CK(Gin CK),Icraiin(Icr) for sperm survive in vitro. Methods：40 sample of male semen were cultured in medium and grouped it in vitro,To adjust the semen concentration is 1.0×107/mL by culture medium of F-10(F-10),To add respect Ginsenoside(Gin1 group,500 μg/mL；Gin2 group,100 μg/mL),Icraiin(Icr1 group,500 μg/mL；Icr2 group,100 μg/mL)were added respectively. F-10 alone was the control groups. To compare with them of groups sperm tmortality. Results：The values of sperm mortality after cultured for 24 h. The motality of I2 group was 41.31±17.49,F group was 47.50±19.85,G2 group was 55.75±24.93,I3 group was 58.05±19.06,G1 group was 98.84±1.62. Ranking them from less to more motality were E2 groups,F groups,I2 groups,G2 groups,I3 groups,G1 group s(p<0.05). Conclusion：When the concentration was 0.1 mg/mL,Gin CK can increase the sperm mortality significantly and Icr was not; When the concentration was 0.5 mg/mL,it is the lethal dose for Gin CK and Icr can increase the sperm mortality but it is not.