Graphene oxide provides an ideal substrate for lysozyme immobilization due to a large specific surface area and abundant functional groups. The lysozyme immobilization on the GO sheets could take place readily by electrostatic interaction of GO-lysozyme (denoted as GO-Ly). The immobilized lysozyme of GO used as the addictive was blended with PES to prepare hybrid ultrafiltration membrane via phase inversion.The effect of GO-Ly content on the morphology, hydrophilic, separation, mechanical and antibacterial properties of hybrid membranes was investigated. The results showed that the surface hydrophilicity and water flux was significantly improved with the increase of GO-Ly content. Meanwhile, the mechanical strength of hybrid membrane was also increased. Especially for 1.5 wt.% of GO-Ly, the water flux reached up to 318 L/(m2?h). The rejection of PVA30000-70000 was 99% and the antibacterial rate of the hybrid membrane against E. coli could achieve 68%.