Based on biological detection and molecular diagnostics technology development, the author summarized high-end equipment independent research in medical diagnostics, disease control, food safety, quarantine inspection, biology and medical research field of molecular diagnostics. The paper focused on research and development of key technology and instrumentation engineering technology, detection performance verification and successful application in the field of social development priorities of the real-time fluorescence quantitative polymerase chain reaction (PCR) nucleic acid detector, diagnosis of high-end equipment, the development and application and subsequent automatic nucleic acid directional monitoring with high-resolution analysis equipment development of handheld adenosine triphosphate (ATP) bioluminescence rapid detection of bacteria.