Based on the steel deck pavement of the Fourth Nanjing Yangtze River Bridge, this paper carried out fatigue performance tests of the pavement at 20 ℃. In order to make the results reflecting the actual situation of pavement more realistically, we used main bridge pavement's mixes for molding specimens; molding and rolling composite beam with steel was at the construction site. The splitting fatigue tests and five stress levels of 0.2, 0.3, 0.4, 0.5, 0.6 were used to study the fatigue property of each layer; in dition, the fatigue tests of composite beams with steel plates and three load levels of 6 kN, 7 kN, 8 kN were chosen to study the fatigue property of the composite structure. Finally, the fatigue equation by regression analysis was got.