In order to choose the suitable grouting material and grouting process, and simulate the actual situation of clearance grouting of rack and nut column in ship lift, grouting process test and prototype grouting experiment were carried out respectively. Comparing three kinds of domestic grouting material and the German PAGEL grouting material in grouting process test, the experimental results showed that the physical and mechanical property indexes of German PAGEL grouting material were superior to the domestic grouting material. In prototype grouting test, selection of the grouting equipment type, mixing time, templates seal, method of grouting parameters control of grouting flow were researched, and some applicable grouting material and grouting process were selected which were suitable for the rack and nut column joint in Three Gorges Project ship lift. The above test effects were obvious, and solved the construction problems of great difficulty in grouting seal and easy blockage of pipeline, etc.