Taking Taizhou Bridge for example, dynamic characteristics of the finite element model are analyzed and then the effects of different moving speeds and loads on dynamic response of three-tower twospan suspension bridge are studied. The result demonstrates that vertical bending of natural frequency is approximately 0.08 Hz, which is far below natural frequency loading truck, and will not form resonance condition. The response of force and displacement of three-tower twospan continuous suspension bridge under simply moving loads and equivalent static loads are the same. The structural will be greater when moving loads quantity and speed become greater and they have similar positive relationship. However, the load amplification factor of structural force and displacement does not vary with the quantity change of moving loads.