The purpose of studying and establishing the regulations is to instruct and standardize the establishment of construction planning of eco-county, eco-city and eco-province. The regulations definitely point out that the essential of eco-county, eco-city and eco-province construction is the capacity construction of sustainable development. The main content includes four aspects： eco-economy, eco-environment, eco-society and eco-culture. Based on expatiating the meaning, significance and requirement of construction planning, the regulations put forward the contents of planning and the procedure, method, means and approval procedure of planning establishment. The regulations can be applied to instructing the establishment of construction planning of eco-county, eco-municipality and eco-province in China. Also the regulations can provide help for censoring construction planning and for examining and assessing construction efficiency.