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王彦吉,赵鹏程,徐媛媛,王景翰
《中国工程科学》 2007年 第9卷 第2期 页码 25-29
讨论了优化电泳条件建立区带毛细管电泳法分析黑色签字笔字迹墨水的分析方法,采用20mmol/L四硼酸钠,pH8.5,未涂层熔融石英毛细管内径100μm,有效柱长40cm,15kV分离电压,光二极管阵列检测器 (PDA),检测波长190~600nm的电泳分离条件,对不同种类黑色签字笔字迹墨水提取液进行了分析;利用毛细管电泳PDA检测器在可见光波长下检测出的黑色签字墨水中不同染料相对含量的变化关系,研究了字迹相对形成时间问题,得出较好的变化规律。
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《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
《工程(英文)》 doi: 10.1016/j.eng.2023.02.016
关键词: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel electrophoresis Mass spectrometry
Simulation on refrigerant flow in adiabatic capillary tube
WANG Meixia, LIU Cunfang, ZHOU Qiangtai
《机械工程前沿(英文)》 2008年 第3卷 第3期 页码 332-336 doi: 10.1007/s11465-008-0054-x
关键词: mathematical diameter literature sub-cooling adiabatic capillary
可去除染料——N-聚糖多方法深入分析中的缺失环节 Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
《工程(英文)》 2023年 第26卷 第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
Mark BOMBERG
《结构与土木工程前沿(英文)》 2010年 第4卷 第4期 页码 431-437 doi: 10.1007/s11709-010-0071-9
关键词: capillary active insulation integrated heating cooling and ventilation air conditioning (HVAC) and building enclosure dynamic insulation switchable thermal resistance variable U-value walls
HE Maogang, ZHANG Ying, SONG Xinzhou, ZHANG Jiantao
《能源前沿(英文)》 2008年 第2卷 第1期 页码 36-42 doi: 10.1007/s11708-008-0013-6
Application of proteomics in environmental science
Xiaona CHU, Jiangyong HU, Say Leong ONG,
《环境科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 393-403 doi: 10.1007/s11783-009-0145-x
关键词: proteomics environmental stress two dimensional (2D) gel electrophoresis mass spectrometry
Genetics analysis of haptoglobin gene in Fujian Han nationality
ZOU Qilian, LIN Ying, LIN Xiangquan, CHEN Yuanzhong
《医学前沿(英文)》 2008年 第2卷 第1期 页码 105-107 doi: 10.1007/s11684-008-0019-9
关键词: different haptoglobin phenotype frequency electrophoresis middle
Purification and characterization of a novel carbonyl reductase with high stereo-selectivity
YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 404-410 doi: 10.1007/s11705-007-0074-9
关键词: asymmetric synthesis sulfate-polyacrylamide purified electrophoresis temperature
Said CHOUCHA, Amar BENYAHIA, Mohamed GHRICI, Mohamed Said MANSOUR
《结构与土木工程前沿(英文)》 2018年 第12卷 第3期 页码 261-269 doi: 10.1007/s11709-017-0394-x
In order to determine the effect of Natural Pozzolan (NP) content on the mechanical properties and durability characteristics on Engineered Cementitious Composites (ECC) as repair material. This study focused on the evaluation of the most factors influencing compatibility between the repair material and the base concrete including mechanicals properties such as, compressive and flexural strengths, elastic modulus, capillary absorption and drying shrinkage. The experimental results showed that natural pozzolan reduces the compressive strength and the flexural strength of ECC at all ages. The elastic modulus of ECC was remarkably lower than that of normal-strength concrete. This lower Young’s modulus is desirable for repair concrete, because it prevents the stresses induced by restrained shrinkage. In addition, the incorporation of high-volume natural pozzolan decreases significantly the coefficient of capillary absorption at long term and increases the drying shrinkage. Generally, based on the results obtained in the present experimental investigation, ECC can be used effectively as an overlay material over existing parent concrete.
关键词: natural pozzolan engineered cementitious composites mechanical strengths elastic modulus capillary absorption drying shrinkage
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
《环境科学与工程前沿(英文)》 2012年 第6卷 第1期 页码 82-97 doi: 10.1007/s11783-011-0306-6
关键词: molecular biological technique microbial community denaturing gradient gel electrophoresis (DGGE) terminal restriction fragment length polymorphism (T-RFLP) environmental applications
《化学科学与工程前沿(英文)》 2023年 第17卷 第5期 页码 548-556 doi: 10.1007/s11705-022-2252-1
关键词: hydrazine-linked TFPB-DHzDS in-situ growth method open-tubular capillary electrochromatography molecular simulations
Investigation of electrochemical degradation and application of e-paper dyes in organic solvents
Luhai LI, Ming WANG, Yi FANG, Shunan QIAO
《化学科学与工程前沿(英文)》 2009年 第3卷 第2期 页码 182-185 doi: 10.1007/s11705-009-0062-3
关键词: electrochemical degradation electrophoresis e-paper oxidized azobenzene group
Manuel J. GOMES, Ning MEI
《能源前沿(英文)》 2020年 第14卷 第1期 页码 114-126 doi: 10.1007/s11708-017-0483-5
关键词: rising liquid film combined flow thermo-capillary flow buoyant flow metallic mesh tube horizontal tube
标题 作者 时间 类型 操作
Simultaneous detection and characterization of toxigenic:Clostridium difficile directly from clinical stool specimens
null
期刊论文
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
期刊论文
Simulation on refrigerant flow in adiabatic capillary tube
WANG Meixia, LIU Cunfang, ZHOU Qiangtai
期刊论文
A concept of capillary active, dynamic insulation integrated with heating, cooling and ventilation, air
Mark BOMBERG
期刊论文
Available energy analysis of new tandem double-capillary tube refrigeration system for refrigerator-freezers
HE Maogang, ZHANG Ying, SONG Xinzhou, ZHANG Jiantao
期刊论文
Genetics analysis of haptoglobin gene in Fujian Han nationality
ZOU Qilian, LIN Ying, LIN Xiangquan, CHEN Yuanzhong
期刊论文
Purification and characterization of a novel carbonyl reductase with high stereo-selectivity
YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong
期刊论文
Effect of natural pozzolan content on the properties of engineered cementitious composites as repair material
Said CHOUCHA, Amar BENYAHIA, Mohamed GHRICI, Mohamed Said MANSOUR
期刊论文
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
期刊论文
Room temperature preparation of hydrazine-linked covalent organic frameworks coated capillaries for separation and determination of polycyclic aromatic hydrocarbons
期刊论文
Investigation of electrochemical degradation and application of e-paper dyes in organic solvents
Luhai LI, Ming WANG, Yi FANG, Shunan QIAO
期刊论文