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马达文,钱静,刘家寿,桂建芳
《中国工程科学》 2016年 第18卷 第3期 页码 96-100 doi: 10.15302/J-SSCAE-2016.03.016
稻田养鱼是一种传统的综合养鱼方式。近几年来,一批以特种经济品种为主导的稻渔综合种养新模式不断涌现,在经济、社会、生态等方面取得显著的成效。为了推广稻渔综合种养的经验,本文实地调研了湖北省两种典型的稻渔综合种养模式,其中“虾–稻共作”模式亩均纯收入超过3 000元,亩纯收入则是单纯种粮的3~4倍;“鳖–虾–鱼–稻共作”模式亩均纯收入近万元,是单一种植水稻亩均效益的12.8倍。本文还提出了加快产业发展的建议,包括进一步拓展种养殖空间、促进产业融合发展及加大政策扶持力度等。
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 90-98 doi: 10.1007/s11705-015-1550-2
关键词: microfluidic chip co-culture cell-cell interactions review
基于液滴微流控的分级结构反蛋白石多孔支架用于仿生三维细胞共培养 Article
邵长敏, 刘羽霄, 池俊杰, 叶方富, 赵远锦
《工程(英文)》 2021年 第7卷 第12期 页码 1778-1785 doi: 10.1016/j.eng.2020.06.031
三维(3D)细胞培养具有更好地模拟天然组织特异性的优势,在药物开发、毒性测试和组织工程中发挥着重要作用。然而,现有的3D细胞培养的支架或微载体通常尺寸有限,并且在模拟生物体内血管复合体方面表现不佳。因此,本研究提出了一种通过简单的微流控方法制备的新型分级结构反蛋白石多孔支架,用于促进3D细胞共培养。该支架是基于微流控乳液液滴模板和惰性聚合物聚合的复合概念构建的。研究结果表明,该支架能够保证细胞培养过程中的营养供给,从而实现大面积的细胞培养。此外,通过在该支架中连续种植不同的细胞,本文还开发了内皮细胞包裹肝细胞的3D细胞共培养系统,用于构建功能化组织。研究结果表明,该支架用于细胞共培养系统,有助于维持肝细胞特定的体内功能。该分级结构反蛋白石多孔支架为3D细胞培养甚至仿生组织的构建奠定了基础。
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
《工程管理前沿(英文)》 2022年 第9卷 第2期 页码 312-325 doi: 10.1007/s42524-020-0135-z
关键词: project culture organisational culture partnering construction culture stakeholder perspectives German
Kishore Gopalakrishnan, Javad Roostaei, Yongli Zhang
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1075-2
RSM is used to explore the impact of different parameter on algal growth response. Mixed algal culture promotes algal biomass and lipid accumulation. Optimized conditions achieve maximum productivity of algal biomass and lipid.
关键词: Algal biofuels Algal mixed cultures Algal biomass Algal lipid Wastewater Response surface methodology
Construction engineering management culture shift: Is the lowest tender offer dead?
Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO
《工程管理前沿(英文)》 2017年 第4卷 第1期 页码 49-57 doi: 10.15302/J-FEM-2017014
关键词: cost certainty lowest responsible bid best value public procurement construction engineering management culture
俞春生,姚蓓,王茜
《中国工程科学》 2009年 第11卷 第3期 页码 92-96
在我国大型工程建设管理的过程中审时度势地融入文化的力量,必将为我国工程建设管理注入活力。工程文化是工程内容与文化形式有机统一的综合体,由于工程的时限性与一次性,使得工程文化体系的建设带有特殊性,通过对苏通大桥工程文化体系建设过程的研究,肯定了大型工程文化建设是工程管理的软实力,同时也在深入分析的基础上,对大型工程建设中构建工程文化体系进行了较为系统的思考,包括工程文化内涵的深刻认识、工程文化建设的原则、工程文化建设的内容以及如何有效地使工程文化快速形成和有效渗透等方面,为后续大型工程文化体系的建设提供了参
关键词: 苏通大桥工程文化 大型工程文化体系建设 系统思维
Studies on culture condition of new marine bacterium sp. SY01
LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua
《化学科学与工程前沿(英文)》 2008年 第2卷 第4期 页码 443-446 doi: 10.1007/s11705-008-0065-5
关键词: producer high-molecular-weight relative culture different culture
俞宁,乔英俊,胡卫国,杨沿平,陈志林,黄威
《中国工程科学》 2018年 第20卷 第1期 页码 139-143 doi: 10.15302/J-SSCAE-2018.01.020
我国进入汽车社会和建设汽车强国迫切需要有先进的汽车文化。本文在分析论述了建设先进汽车文化重要性的基础上,对我国汽车文化的现状及存在的问题进行了分析,明确了我国建设先进汽车文化的目标及指导思想,提出了发展先进汽车文化的路径,这些都将有利于我国汽车文化的发展壮大。
Jiuxiao Hao, Xiujin Wang, Hui Wang
《环境科学与工程前沿(英文)》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0896-8
关键词: Polyhydroxyalkanoates (PHAs) Valerate Mixed culture 3-hydroxyvalerate (3HV) Propionate
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
《医学前沿(英文)》 2019年 第13卷 第4期 页码 492-503 doi: 10.1007/s11684-019-0681-0
关键词: haploidentical hematopoietic stem cell transplantation stool culture Candida
The
Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA
《化学科学与工程前沿(英文)》 2012年 第6卷 第2期 页码 152-157 doi: 10.1007/s11705-012-1287-0
关键词:
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
Long LIU, Haiquan YANG, Guocheng DU, Miao WANG, Jian CHEN,
《化学科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 351-356 doi: 10.1007/s11705-009-0248-8
标题 作者 时间 类型 操作
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
期刊论文
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
期刊论文
Construction engineering management culture shift: Is the lowest tender offer dead?
Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO
期刊论文
Studies on culture condition of new marine bacterium sp. SY01
LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua
期刊论文
Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant
Jiuxiao Hao, Xiujin Wang, Hui Wang
期刊论文
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
期刊论文
The
Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA
期刊论文
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue cultures
null
期刊论文