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bioconversion 2

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cholesterol oxidase 1

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Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 3,   Pages 615-629 doi: 10.1007/s11705-020-1989-7

Abstract: A cholesterol oxidase (COD) was hybridized with Ca , Zn , Al , Fe and Mn . After precipitation with PO at 4 °C for 72 h, the resulting pellets were freeze-dried. In scanning electron microscopy assays, the metal-COD complexes revealed flower-like or granular structures after hybridization. Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials. X-ray diffraction analysis indicated that COD was encapsulated in CaHPO ·2H O-, Zn (PO ) ·4H O-, AlPO -, FeP - and Mn (PO ) ·3H O-based nanostructures, respectively. Differential scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5 °C to 167.02 °C, 167.02 °C, 137.70 °C, 172.85 °C and 160.99 °C, respectively. Using steroid derivatives as substrates, this enzyme could convert cholesterol, pregnenolone, dehydroepiandrosterone, ergosterol, -sitosterol and stigmasterol to related single products. Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme. In addition, substrate selectivity could be affected by various metal materials. Briefly, using Ca , Zn , Al , Fe and Mn as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures, and could help to promote the stability and tolerant properties of the enzyme, while also enhancing its catalytic characteristics.

Keywords： cholesterol oxidase     metal-enzyme hybridization     nanostructures     sterol derivatives     bioconversion    

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Two-phase Partitioning Bioreactor, Application of Cloud Point System in Bioconversion

Wang Zhilong

Strategic Study of CAE 2005, Volume 7, Issue 5,   Pages 73-78

Abstract: enhancing the substrate solubility and eliminating the substrate inhibition of bioprocesses, such as bioconversionpoint system as a novel two-phase partitioning bioreactor is illustrated by reference to a particular bioconversion

Keywords： two-phase partitioning     bioconversion     nonionic surfactant     cloud point system     bioreactor    

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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 772-779 doi: 10.1007/s11705-018-1731-x

Abstract: We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) fromT7- was constructed and transformed to BL21(DE3) to obtain the whole cells of BL21- capable of bioconversion

Keywords： D-1     2     4-butanetriol     whole-cell bioconversion     carbon source     nitrogen sources     metal ions     culture conditions    

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From Discovery to Mass Production: A Perspective on Bio-Manufacturing Exemplified by the Development of Statins Review

Xiaoling Tang, Jiawei Yu, Yuheng Geng, Jiarui Wang, Renchao Zheng, Yuguo Zheng

Engineering 2023, Volume 24, Issue 5,   Pages 138-150 doi: 10.1016/j.eng.2022.04.030

Abstract: production of the first-generation statins from microorganisms, the second-generation statins using bioconversion

Keywords： Bio-manufacturing     Statins     Microorganisms     Bioconversion     Chemoenzymatic synthesis    

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Title Author Date Type Operation

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Journal Article

Two-phase Partitioning Bioreactor, Application of Cloud Point System in Bioconversion

Wang Zhilong

Journal Article

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Journal Article

From Discovery to Mass Production: A Perspective on Bio-Manufacturing Exemplified by the Development of Statins

Xiaoling Tang, Jiawei Yu, Yuheng Geng, Jiarui Wang, Renchao Zheng, Yuguo Zheng

Journal Article