Effects of Co2+ on the erythromycin biosynthesis

2007年第1卷第3期

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《化学科学与工程前沿（英文）》 >> 2007年第1卷第3期 doi: 10.1007/s11705-007-0044-2

Effects of Co2+ on the erythromycin biosynthesis

1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; College of Bioengineering, East China University of Science and Technology, Shanghai 200237, China;

发布日期： 2007-09-05

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摘要

Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism. The specific activities of the key enzymes related to glucose metabolism such as hexose kinase (HK), glucose-6-phosphate dehydrogenase (6-PDH), phosphofructokinase (PFK), and isocitrate dehydrogenase (ICD), were determined in . The specific activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase (MCM) and methylmalonyl-coenzyme A transcarboxylase (MCT), were detected as well. Some organic acids contained in fermentation broth were also analyzed. The results show that Co is able to increase erythromycin biosynthesis. It maybe due to Co improving the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcarboxylase. Meanwhile, it also enhances the flux of the glucose metabolism pathway.

关键词

secondary metabolism ; organic ; fermentation ; process ; phosphofructokinase