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Prevalence and drug resistance characteristics of carbapenem-resistant Enterobacteriaceae in Hangzhou

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《医学前沿（英文）》 2018年第12卷第2期页码 182-188 doi: 10.1007/s11684-017-0529-4

摘要：

With the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant Enterobacteriaceae (CRE) attracted considerable public concern. A retrospective study was conducted based on 242 CRE strains from a tertiary hospital in Hangzhou, China to investigate prevalence and drug resistance characteristics of CRE in southeast China. Bacterial species were identified. Antimicrobial susceptibility was examined by broth microdilution method or epsilometer test. Resistant β-lactamase genes were identified by polymerase chain reaction and sequencing. Genotypes were investigated by phylogenetic analysis. and were the most prevalent types of species, with occurrence in 71.9% and 21.9% of the strains, respectively. All strains exhibited high resistance (>70%) against β-lactam antibiotics, ciprofloxacin, trimethoprim–sulfamethoxazole, and nitrofurantoin but exhibited low resistance against tigecycline (0.8%) and minocycline (8.3%). A total of 123 strains harbored more than two kinds of β-lactamase genes. , , , and were the predominant genotypes, with detection rates of 60.3%, 61.6%, 43.4%, and 16.5%, respectively, and were highly identical with reference sequences in different countries, indicating potential horizontal dissemination. IMP-4 was the most frequent class B metallo-lactamases in this study. In conclusion, continuous surveillance and effective prevention should be emphasized to reduce spread of CRE.

关键词： Enterobacteriaceae carbapenem β-lactamase genes phylogenetic analysis

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Characterization of NDM-1-producing carbapenemase in

Rongmin ZHANG,Yang WANG,Zhihai LIU,Jiyun LI,Wenjuan YIN,Lei LEI,Congming WU,Jianzhong SHEN

《农业科学与工程前沿（英文）》 2015年第2卷第3期页码 223-229 doi: 10.15302/J-FASE-2015065

摘要： In recent years, the mobile metallo-β-lactamase (MBL) genes have been found to correspond to one of the most important resistance characters identified in Gram-negative bacteria, severely affecting clinical chemotherapy and threatening public health. The prevalence of mobile MBL genes and their flanking regions in Gram-negative bacteria from diseased pigs in China was investigated. A total of 334 lung samples from diseased pigs were screened for Gram-negative bacteria classified as non-susceptible to meropenem (MIC≥4 mg·L ). Six isolates, including three , two and one , exhibited MBL production and carried the gene. S1-PFGE and Southern blot analysis showed that the gene was located on the chromosome of one isolate and on plasmids of various sizes in the other five isolates. MIC testing using broth microdilution revealed that all -carrying isolates and some of their transconjugants exhibited resistance to almost all β-lactams tested. Whole genome sequencing revealed that the flanking region of the gene from all porcine isolates had high levels of similarity with the corresponding regions in human isolates. One porcine isolate carrying was typed as ST48, a common sequence type in human isolates. These results suggest the possibility of human-to-food animal transfer of -producing , highlighting the need for surveillance of carbapenemase producers among bacteria from food animals. In addition, the prudent use of antimicrobial agents to decrease the opportunities for co-selection of carbapenemase genes in food animals is also urgently needed.

关键词： carbapenemase NDM-1 ISAba125 Enterobacteriaceae food safety