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Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE
《环境科学与工程前沿（英文）》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0846-x
关键词： DNA microarray analysis Nitrogen cycling functional genes Most probable number-polymerase chain reaction (MPN-PCR) Wastewater treatment plants (WWTPs)
《农业科学与工程前沿（英文）》 2022年 第9卷 第3期 页码 407-424 doi: 10.15302/J-FASE-2022448
● Soil nitrogen fluxes and influencing factors were reviewed in the subtropical hilly regions.
关键词： nitrogen cycling soil nitrogen nitrogen deposition greenhouse gases emission non-point source pollution nitrogen use efficiency
Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI
《环境科学与工程前沿（英文）》 2012年 第6卷 第6期 页码 797-805 doi: 10.1007/s11783-012-0405-z
关键词： crude oil-contaminated soil phytoremediation rhizosphere effects rhizobox functional genes
Overlooked nitrogen-cycling microorganisms in biological wastewater treatment
《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1426-2
• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.
关键词： Ammonia oxidizing archaea (AOA) Complete ammonia oxidizing (comammox) bacteria Dissimilatory nitrate reduction to ammonium (DNRA) bacteria Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms Engineering application
基于宏基因组分析揭示焦化废水处理系统中微生物群落的结构与功能——基于脱氮基因 Article
Jiaying Ma, Fan Wang, Haifeng Fan, Enchao Li, Huaqiang Chu, Xuefei Zhou, Yalei Zhang
《工程（英文）》 2024年 第36卷 第5期 页码 75-86 doi: 10.1016/j.eng.2023.06.019
Microbial communities play crucial roles in pollutant removal and system stability in biological systems for coking wastewater (CWW) treatment, but a comprehensive understanding of their structure and functions is still lacking. A five-month survey of four sequential bioreactors, anoxic 1/oxic 1/anoxic 2/oxic 2 (A1/O1/A2/O2), was carried out in a full-scale CWW treatment system in China to elucidate operational performance and microbial ecology. The results showed that A1/O1/A2/O2 had excellent and stable performance for nitrogen removal. Both total nitrogen (TN; (17.38 ± 6.89) mg·L−1) and ammonium–nitrogen (NH4+-N; (2.10 ± 1.34) mg·L−1) in the final biological effluent satisfied the Chinese national standards for CWW. Integrated analysis of 16S ribosome RNA (rRNA) sequencing and metagenomic sequencing showed that the bacterial communities and metagenomic function profiles of A1 and O1 shared similar functional structures, while those of A2 significantly varied from those of other bioreactors (p < 0.05). The results indicated that microbial activity was strongly connected with activated sludge function. Nitrosospira, Nitrosomonas, and SM1A02 were responsible for nitrification during the primary anoxic–oxic (AO) stage and Azoarcus and Thauera acted as important denitrifiers in A2. Nitrogen cycling-related enzymes and genes work in the A1/O1/A2/O2 system. Moreover, the hao genes catalyzing hydroxylamine dehydrogenase (EC 1.7.2.6) and the napA and napB genes catalyzing nitrate reductase (EC 1.9.6.1) played important roles in the nitrification and denitrification processes in the primary and secondary AO stages, respectively. The mixed liquor suspended solids (MLSS)/total solids (TS), TN removal rate, total organic carbon (TOC) removal rate, and NH4+-N removal rate were the most important environmental factors for regulating the structure of core bacterial genera and nitrogen-cycling genes. Proteobacteria were the potential main participants in nitrogen metabolism in the A1/O1/A2/O2 system for CWW treatment. This study provides an original and comprehensive understanding of the microbial community and functions at the gene level, which is crucial for the efficient and stable operation of the full-scale biological process for CWW treatment.
关键词： Coking wastewater Full-scale Microbial community Metagenomic sequencing Nitrogen-cycling genes Environmental factor
《农业科学与工程前沿（英文）》 2022年 第9卷 第2期 页码 177-186 doi: 10.15302/J-FASE-2021421
A range of plant species produce root exudates that inhibit ammonia-oxidizing microorganisms. This biological nitrification inhibition (BNI) capacity can decrease N loss and increase N uptake from the rhizosphere. This study sought evidence for the existence and magnitude of BNI capacity in canola ( Brassica napus). Seedlings of three canola cultivars, Brachiaria humidicola(BNI positive) and wheat ( Triticum aestivum) were grown in a hydroponic system. Root exudates were collected and their inhibition of the ammonia oxidizing bacterium, Nitrosospira multiformis, was tested. Subsequent pot experiments were used to test the inhibition of native nitrifying communities in soil. Root exudates from canola significantly reduced nitrification rates of both N. multiformis cultures and native soil microbial communities. The level of nitrification inhibition across the three cultivars was similar to the well-studied high-BNI species B. humidicola. BNI capacity of canola may have implications for the N dynamics in farming systems and the N uptake efficiency of crops in rotational farming systems. By reducing nitrification rates canola crops may decrease N losses, increase plant N uptake and encourage microbial N immobilization and subsequently increase the pool of organic N that is available for mineralization during the following cereal crops.
TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo
《医学前沿（英文）》 2007年 第1卷 第1期 页码 41-45 doi: 10.1007/s11684-007-0008-4
《农业科学与工程前沿（英文）》 2022年 第9卷 第2期 页码 245-271 doi: 10.15302/J-FASE-2021439
Agricultural intensification and the subsequent decline of mixed farming systems has led to an increase in continuous cropping with only a few fallow or break years, undermining global soil health. Arable-ley rotations incorporating temporary pastures (leys) lasting 1–4 years may alleviate soil degradation by building soil fertility and improving soil structure. However, the majority of previous research on arable-ley rotations has utilized either grass or grass-clover leys within ungrazed systems. Multispecies leys, containing a mix of grasses, legumes, and herbs, are rapidly gaining popularity due to their promotion in agri-environment schemes and potential to deliver greater ecosystem services than conventional grass or grass-clover leys. Livestock grazing in arable-ley rotations may increase the economic resilience of these systems, despite limited research of the effects of multispecies leys on ruminant health and greenhouse gas emissions. This review aims to evaluate previous research on multispecies leys, highlighting areas for future research and the potential benefits and disbenefits on soil quality and livestock productivity. The botanical composition of multispecies leys is crucial, as legumes, deep rooted perennial plants (e.g., Onobrychis viciifolia and Cichorium intybus) and herbs (e.g., Plantago lanceolata) can increase soil carbon, improve soil structure, reduce nitrogen fertilizer requirements, and promote the recovery of soil fauna (e.g., earthworms) in degraded arable soils while delivering additional environmental benefits (e.g., biological nitrification inhibition and enteric methane reduction). Multispecies leys have the potential to deliver biologically driven regenerative agriculture, but more long-term research is needed to underpin evidence-based policy and farmer guidance.
MANURE AND METROPOLES---A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS
《农业科学与工程前沿（英文）》 2021年 第8卷 第1期
Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang
《环境科学与工程前沿（英文）》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1062-7
Effects of urban river conditions on phytoplankton community were investigated. Nitrate utilization and hydrodynamic condition affected phytoplankton distribution. A winter bloom was induced by abundant ammonia, total phosphorus and organic matters. The diatoms and euglenoids dominated in an urban beheaded river ecosystem.
关键词： Phytoplankton composition Spatiotemporal distribution Functional groups Nitrate nitrogen Winter bloom
Metagenomic analysis on resistance genes in water and microplastics from a mariculture system
《环境科学与工程前沿（英文）》 2022年 第16卷 第1期 页码 4-4 doi: 10.1007/s11783-021-1438-y
Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.
关键词： Antibiotic resistance genes Metal resistance genes Metagenomic analysis Microplastics Mariculture
MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS
Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF
《农业科学与工程前沿（英文）》 2021年 第8卷 第1期 页码 182-187 doi: 10.15302/J-FASE-2020364
Occurrence and possible sources of antibiotic resistance genes in seawater of the South China Sea
《环境科学与工程前沿（英文）》 doi: 10.1007/s11783-024-1868-4
● The highest absolute abundance of ARGs in seawater reached 2.3 × 104 copies/mL.
关键词： The South China Sea Antibiotic resistance genes Mobile genetic element Source apportionment
Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes
Daniel GAONA,Alfredo VALAREZO
《机械工程前沿（英文）》 2015年 第10卷 第3期 页码 294-300 doi: 10.1007/s11465-015-0351-0
The elastic modulus of a deposit (Ed) can be obtained by monitoring the temperature (?T) and curvature (?k) of a one-side coated long plate, namely, a one-dimensional (1D) deformation model. The aim of this research is to design an experimental setup that proves whether a 1D deformation model can be scaled for complex geometries. The setup includes a laser displacement sensor mounted on a robotic arm capable of scanning a specimen surface and measuring its deformation. The reproducibility of the results is verified by comparing the present results with Stony Brook University Laboratory’s results. The ?k-?T slope error is less than 8%, and the Ed estimation error is close to 2%. These values reveal the repeatability of the experiments. Several samples fabricated with aluminum as the substrate and 100MXC nanowire (Fe and Cr alloy) as the deposit are analyzed and compared with those in finite element (FE) simulations. The linear elastic behavior of 1D (flat long plate) and 2D (squared plate) specimens during heating/cooling cycles is demonstrated by the high linearity of all ?k-?T curves (over 97%). The Ed values are approximately equal for 1D and 2D analyses, with a median of 96 GPa and standard deviation of 2 GPa. The correspondence between the experimental and simulated results for the 1D and 2D specimens reveals that deformation and thermal stress in coated specimens can be predicted regardless of specimen geometry through FE modeling and by using the experimental value of Ed. An example of a turbine-blade-shaped substrate is presented to validate the approach.
关键词： in-plane Young’s modulus curvature temperature thermal stress coating
Mengli Wang, Ruying Li, Qing Zhao
《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2
Sludge digestion is critical to control the spread of ARGs from wastewater to soil. Fate of ARGs in three pretreatment-AD processes was investigated. UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process. The total ARGs concentration showed significant correlation with 16S rRNA gene. The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.
关键词： Tetracycline resistance genes Sulfonamide resistance genes 16S rRNA gene Sludge pretreatment Anaerobic sludge digestion
标题 作者 时间 类型 操作
Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray
Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE
期刊论文
SOIL NITROGEN CYCLING AND ENVIRONMENTAL IMPACTS IN THE SUBTROPICAL HILLY REGION OF CHINA: EVIDENCE FROM
期刊论文
Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil
Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI
期刊论文
基于宏基因组分析揭示焦化废水处理系统中微生物群落的结构与功能——基于脱氮基因
Jiaying Ma, Fan Wang, Haifeng Fan, Enchao Li, Huaqiang Chu, Xuefei Zhou, Yalei Zhang
期刊论文
ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING AMMONIA OXIDATION IN SOIL
期刊论文
Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes
TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo
期刊论文
AGRONOMIC AND ENVIRONMENTAL BENEFITS OF REINTRODUCING HERB- AND LEGUME-RICH MULTISPECIES LEYS INTO ARABLE ROTATIONS: A REVIEW
期刊论文
Distribution of phytoplankton community and its influence factors in an urban river network, East China
Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang
期刊论文
MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS
Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF
期刊论文
Occurrence and possible sources of antibiotic resistance genes in seawater of the South China Sea
期刊论文
Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes
Daniel GAONA,Alfredo VALAREZO
期刊论文
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