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生物传感器 3

临床决策支持系统 1

人体传感网络 1

体液免疫应答 1

即时医疗 1

可植入传感器 1

合成生物学 1

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外泌体 1

大数据 1

移动医疗 1

结直肠癌 1

肿瘤微环境 1

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药物遗传学 1

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Advances in airborne microorganisms detection using biosensors: A critical review

《环境科学与工程前沿（英文）》 2021年第15卷第3期 doi: 10.1007/s11783-021-1420-8

摘要：

Humanity has been facing the threat of a variety of infectious diseases. Airborne microorganisms can cause airborne infectious diseases, which spread rapidly and extensively, causing huge losses to human society on a global scale. In recent years, the detection technology for airborne microorganisms has developed rapidly; it can be roughly divided into biochemical, immune, and molecular technologies.

关键词： Biosensor Airborne microorganisms Microbiological detection technology

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Anti-biofouling strategies for implantable biosensors of continuous glucose monitoring systems

《化学科学与工程前沿（英文）》 2023年第17卷第12期页码 1866-1878 doi: 10.1007/s11705-023-2340-x

摘要： Continuous glucose monitoring (CGM) systems play an increasingly vital role in the glycemic control of patients with diabetes mellitus. However, the immune responses triggered by the implantation of poorly biocompatible sensors have a significant impact on the accuracy and lifetime of CGM systems. In this review, research efforts over the past few years to mitigate the immune responses by enhancing the anti-biofouling ability of sensors are summarized. This review divided these works into active immune engaging strategy and passive immune escape strategy based on their respective mechanisms. In each strategy, the various biocompatible layers on the biosensor surface, such as drug-releasing membranes, hydrogels, hydrophilic membranes, anti-biofouling membranes based on zwitterionic polymers, and bio-mimicking membranes, are described in detail. This review, therefore, provides researchers working on implantable biosensors for CGM systems with vital information, which is likely to aid in the research and development of novel CGM systems with profound anti-biofouling properties.

关键词： implantable glucose biosensor anti-biofouling continuous glucose monitoring immune responses

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Genetic biosensors for small-molecule products: Design and applications in high-throughput screening

Qingzhuo Wang,Shuang-Yan Tang,Sheng Yang

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 15-26 doi: 10.1007/s11705-017-1629-z

摘要： Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into biosensors of small molecules, which couple the intracellular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screening of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.

关键词： biosensor small molecule product transcription factor riboswitch high-throughput screening

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用于个性化医疗的植入式生物传感器展望

Rita Rebelo,Ana Isabel Barbosa,Vitor M. Correlo,Rui L. Reis

《工程（英文）》 2021年第7卷第12期页码 1696-1699 doi: 10.1016/j.eng.2021.08.010

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oocyst directed assembly of gold nanoparticles and graphene oxide

Sona Jain, Zhicheng Huang, Brent R. Dixon, Syed Sattar, Juewen Liu

《化学科学与工程前沿（英文）》 2019年第13卷第3期页码 608-615 doi: 10.1007/s11705-019-1813-4

摘要： Understanding the interactions between inorganic nanomaterials and biological species is an important topic for surface and environmental chemistry. In this work, we systematically studied the oocysts of as a model protozoan parasite and its interaction with gold nanoparticles (AuNPs) and graphene oxide (GO). The as-prepared citrate-capped AuNPs adsorb strongly on the oocysts leading to a vivid color change. The adsorption of the AuNPs was confirmed by transmission electron microscopy. Heat treatment fully inhibited the color change, indicating a large change of surface chemistry of the oocysts that can be probed by the AuNPs. Adding proteases such as trypsin and proteinase K partially inhibited the color change. DNA-capped AuNPs, on the other hand, could not be adsorbed by the oocysts. GO was found to wrap around the oocysts forming a conformal shell reflecting the shape of the oocysts. Both citrate-capped AuNPs and GO compromised the membrane integrity of the oocysts as indicated by the propidium iodide staining experiment, and they may be potentially used for inactivating the oocysts. This is the first example of using nanomaterials to probe the surface of the oocysts, and it suggests the possibility of using such organisms to template the assembly of nanomaterials.

关键词： nanomaterials toxicology water biosensors

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大数据与精准医学

Daniel Richard Leff, Guang-Zhong Yang

《工程（英文）》 2015年第1卷第3期页码 277-279 doi: 10.15302/J-ENG-2015075

摘要：

本文主要关注大数据分析对改善健康、预防和检测早期疾病以及个性化干预措施的潜在影响，讨论了大数据分析在建立患者电子健康档案以获得更好的临床决策支持中的可能作用。笔者研究了药物遗传学的发展，以更好地理解患者对化学疗法产生不同反应的原因。此外，笔者评估了在线健康交流的发展，以及如何利用这些数据检测公共健康威胁和控制传染病。最后，笔者描述了新一代可穿戴和可植入的躯体传感器如何改善身体状况、简化慢性病管理和提高外科植入物质量。

关键词：大数据生物传感器人体传感网络可植入传感器临床决策支持系统药物遗传学移动医疗

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一种可实现合成生物传感器现场部署的增材制造方法 Article

Daniel Wolozny, John R. Lake, Paul G. Movizzo, Zhicheng Long, Warren C. Ruder

《工程（英文）》 2019年第5卷第1期页码 173-180 doi: 10.1016/j.eng.2018.12.001

摘要：

合成生物学工具可用于设计活体生物传感器，报告目标分析物的存在。虽然这些工程细胞生物传感器在实验室外具有许多潜在应用，但由于它们属于转基因生物（genetically modified organism，GMO），通常被认为具有危险性。因此，如何在实验室外使用转基因生物的同时，降低将其释放到环境中的风险就至关重要。本文描述了一种包含合成生物电路的生物传感系统。含有该系统的大肠杆菌（Escherichia coli）被置于一个特制的3D 打印的试管盖内。这些转基因生物能够检测到一种条件致病菌铜绿假单胞菌（Pseudomonas aeruginosa）的化学群体信号。在该设备中，活体生物传感器可以在不接触环境的情况下，接触感兴趣的样本。细胞可以在培养管内进行现场可视化分析，也可以送回实验室进行进一步分析。许多生物传感器缺乏现场部署所需的多功能性，由于
缺乏资源和装置，许多疾病可能无法诊断。我们的生物检测设备利用3D 打印技术，为现场部署活体生物传感器制造了一种便携式、模块化和廉价的设备。

关键词：合成生物学增材制造生物传感器

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从结直肠癌细胞和组织样本释放的外泌体中检测肿瘤相关抗原自身抗体的多路生物传感诊断平台展示了对结直肠癌的高诊断能力 Article

Ana Montero-Calle, Itziar Aranguren-Abeigon, María Garranzo-Asensio, Carmen Poves, María Jesús Fernández-Aceñero, Javier Martínez-Useros, Rodrigo Sanz, Jana Dziaková, Javier Rodriguez-Cobos, Guillermo Solís-Fernández, Eloy Povedano, Maria Gamella, Rebeca Magnolia Torrente-Rodríguez, Miren Alonso-Navarro, Vivian de los Ríos, J. Ignacio Casal, Gemma Domínguez, Ana Guzman-Aranguez, Alberto Peláez-García, José Manuel Pingarrón, Susana Campuzano, Rodrigo Barderas

《工程（英文）》 2021年第7卷第10期页码 1393-1412 doi: 10.1016/j.eng.2021.04.026

摘要：

结直肠癌（CRC）是全球导致癌症相关死亡的第二大原因。CRC患者的5年生存率与诊断分期息息相关。CRC早期诊断患者的5年生存率高于80%，CRC晚期诊断患者的5年生存率低于10%。大量研究表明，患者血清中特定CRC自身抗原[肿瘤相关抗原（TAA）]对应的自身抗体有助于早期诊断。因此，本文旨在识别CRC特异性自身抗体的自身抗原靶点，借助液体活检技术有效筛查CRC患者和健康个体。为此，我们从CRC患者体内提取5个CRC细胞系和组织样本，通过免疫沉淀-质谱分析法测量其分泌的外泌体的蛋白质含量，共鉴定出103种蛋白质为潜在的CRC特异性自身抗原。采用生物信息学技术和荟萃分析，我们选定了15种与实际CRC自身抗原类似度更高的蛋白质，以便后续借助蛋白质印迹法（WB）和免疫组织化学技术（IHC）评估它们在CRC预后中的作用。结果发现，在患者的组织和血浆外泌体样本中，有11种蛋白质发生蛋白质水平失调，有9种蛋白质与CRC预后有关。经验证发现，除一例外，所有研究均显示出具有统计学意义的高诊断能力，采用荧光Halotag磁珠，或者借助多路生物传感平台（磁性微载体为固相载体，由用于CRC细胞检测的共价固定Halotag融合蛋白修饰），能够有效筛查区分CRC患者、癌前病变个体与健康个体。综上所述，本文的研究结果突出了此研究方法在识别慢性疾病特征TAA时的有效性；此外，本文采用的测量血浆中TAA对应的自身抗体水平的方法可以应用到具有高诊断能力的CRC检测即时医疗（POC）设备中。

关键词：自身抗体诊断结直肠癌外泌体肿瘤微环境体液免疫应答即时医疗生物传感器