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Preparation of hemicellulolic oligosaccharides from

Yuya KUMAGAI, Hirokazu USUKI, Yukihiro YAMAMOTO, Akihiro YAMASATO, Takafumi MUKAIHARA, Tadashi HATANAKA

《化学科学与工程前沿（英文）》 2012年第6卷第2期页码 224-231 doi: 10.1007/s11705-012-1280-7

摘要： Wood biomass is anticipated to serve as a substitute for carbon source, which has no feedstock competition with foods. Biomass is commonly used for the production of bio-ethanol by a series of processes such as pretreatment, enzymatic degradation, and fermentation. Hemicellulose, constituting 20 wt-% – 40 wt-% of biomass materials, contains various kinds of saccharides known to be bioactive substrates. Practical usage of hemicellulose is generally limited to its conversion to bio-ethanol. Here, we aimed to prepare hemicellulolic oligosaccharides, more valuable products other than ethanol. Therefore, the Hinoki slurry was treated with lime at room temperature for 3 h, and then neutralized with HCl. The resulting sample was treated with 13 types of commercial enzymes, and the saccharides produced in the supernatant were evaluated. The result showed that the commercial enzyme Cellulase SS (Nagase & Co., LTD.) effectively degraded the slurry to produce disaccharides and trisaccharides. Analysis of sugar components by liquid chromatography/mass spectrography (LC/MS) after the derivation with ethyl 4-aminobenzoate (ABEE) showed that mannobiose, mannotriose, and cellobiose were the major oligosaccharides. These results indicate valuable oligosaccharides can be successfully produced from Hinoki softwood slurry.

关键词： hemicellulolic oligosaccharides Chamaecyparis obtuse (Hinoki) commercial enzyme manno-oligosaccharide cello-oligosaccharide

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Experimental study and product analysis of lignocellulosic biomass hydrolysis under extremely low acids

WANG Shurong, LUO Zhongyang, CEN Kefa, ZHUANG Xinshu

《能源前沿（英文）》 2008年第2卷第3期页码 268-272 doi: 10.1007/s11708-008-0046-x

摘要： Using our self-designed facility for lignocellulosic biomass hydrolysis under extremely low acid, and under the optimal reaction conditions of hemicellulose and cellulose determined by xylan and quantitative filter paper as the model, two-step dilute acid hydrolysis was carried out in detail on pine, fast-growing poplar and maize straw. The corresponding conversion ratio is 41.78%, 57.84% and 53.44% and the total reducing sugar conversion ratio is 39.28%, 42.83% and 23.82% respectively. Simultaneously, sugar component analysis of the products was performed by high performance liquid chromatography (HPLC) and monosaccharide and oligosaccharide content were compared. Finally, poplar hydrolysis residues were analyzed by proximate and ultimate analysis.

关键词： HPLC oligosaccharide lignocellulosic chromatography reducing