资源类型

期刊论文 1

年份

2007 1

关键词

检索范围：

排序： 展示方式：

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

《化学科学与工程前沿（英文）》 2007年 第1卷 第4期   页码 399-403 doi: 10.1007/s11705-007-0073-x

摘要： The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×10 U/mL, respectively.

关键词： bioactivity     pastoris     maximum     induction     optimized    

HTML PDF 收藏

标题 作者 时间 类型 操作

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

期刊论文