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期刊论文 1

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2009 1

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Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

《化学科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 393-398 doi: 10.1007/s11705-009-0252-z

摘要: -psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of -psicose from -fructose. In this paper, another bacterium which could convert -fructose to -psicose was isolated and identified as . The process parameters of -psicose production using permeabilized cells of SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10min, and reaction conditions: cell concentration, 30g dry cell wt/L; concentration of substrate, 50g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5g/L -psicose with a -psicose productivity of 0.82g·L·h. This is the first report of bioproduction of -psicose using permeabilized cells of .

关键词: reaction     permeabilized     substrate     bacterium     process    

标题 作者 时间 类型 操作

Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

期刊论文