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Reconsideration on the effect of nitrogen on mixed culture polyhydroxyalkanoate production toward high

Zhiqiang Chen, Lizhi Zhao, Ye Ji, Qinxue Wen, Long Huang

《环境科学与工程前沿（英文）》 2019年 第13卷 第4期 doi: 10.1007/s11783-019-1135-2

摘要：

Effect of nitrogen on mixed culture PHA production was reconsidered.

Enrichment history of PHA accumulating culture was discussed.

Higher PHA content and biomass growth were achieved in presence of nitrogen.

Enrichment strategy toward higher PHA accumulation was investigated.

Microbial community succession in PHA accumulation phase was investigated.

关键词： Polyhydroxyalkanoate (PHA)     Organic loading rate     Nitrogen content     Biomass growth     Enrichment history    

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Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

《化学科学与工程前沿（英文）》 2017年 第11卷 第1期   页码 139-142 doi: 10.1007/s11705-017-1636-0

摘要： Establishment of the regeneratable whole-cell catalyst platform for the?production of biobased polymeric materials is a?typical topic of synthetic biology. In this commentary, discovery story of a “lactate-polymerizing enzyme” (LPE)?and LPE-based?achievements for creating a new variety of polyesters with incorporated unnatural monomers are presented. Besides the importance of microbial platform itself is discussed referring to the “ballooning”- .

关键词： synthetic biology     enzyme evolutionary engineering     polyhydroxyalkanoate    

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Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

《环境科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 616-624 doi: 10.1007/s11783-013-0505-4

摘要： The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifying phosphorus (P) removal performance and N O production was examined using a denitrifying enhanced biologic phosphorus removal (EBPR) sludge acclimatized with mixed acetate (HAc) and propionate (Pro) (in the molar ratio 3∶1) as carbon sources. The results showed that when the AnRT was prolonged from 90 to 150 min, the anaerobic polyhydroxyalkanoate (PHA) synthesis was decreased by 15.3%. Moreover, the ineffective PHA consumption occurred in anaerobic phases and contributed to an increased accumulation and higher free nitrous acid (FNA) concentrations (≥0.001–0.0011 mg HNO -N/L) in the subsequent anoxic phases, causing a severe inhibition on anoxic P-uptake and denitrification. Accordingly, the total nitrogen (TN) and total phosphorus (TP) removal efficiencies dropped by approximately 6.3% and 85.5%, respectively; and the ratio of anoxic N O-N production to TN removal increased by approximately 3.8%. The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated by (62.0% (SE = 1.5%)). In conclusion, the short-term excessive anaerobic reaction time negatively impacted denitrifying P removal performance and stimulated more N O production, and its effect on P removal was more obvious than that on nitrogen removal.

关键词： Denitrifying phosphorus removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescence in-situ hybridization    

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标题 作者 时间 类型 操作

Reconsideration on the effect of nitrogen on mixed culture polyhydroxyalkanoate production toward high

Zhiqiang Chen, Lizhi Zhao, Ye Ji, Qinxue Wen, Long Huang

期刊论文

Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

期刊论文

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

期刊论文