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Identification of dynamic stiffness matrix of bearing joint region

Feng HU, Bo WU, Youmin HU, Tielin SHI

《机械工程前沿（英文）》 2009年第4卷第3期页码 289-299 doi: 10.1007/s11465-009-0064-3

摘要： The paper proposes an identification method of the dynamic stiffness matrix of a bearing joint region on the basis of theoretical analysis and experiments. The author deduces an identification model of the dynamic stiffness matrix from the synthetic substructure method. The dynamic stiffness matrix of the bearing joint region can be identified by measuring the matrix of frequency response function (FRFs) of the substructure (axle) and whole structure (assembly of the axle, bearing, and bearing housing) in different positions. Considering difficulty in measuring angular displacement, applying moment, and directly measuring relevant FRFs of rotational degree of freedom, the author employs an accurately calibrated finite element model of the unconstrained structure for indirect estimation. With experiments and simulation analysis, FRFs related with translational degree of freedom, which is estimated through the finite element model, agrees with experimental results, and there is very high reliability in the identified dynamic stiffness matrix of the bearing joint region.

关键词： frequency response function (FRFs) dynamic stiffness finite element synthetic substructure method joint region

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基于子结构分析的多重子步模型修正方法

郭力,李兆霞,陈鸿天

《中国工程科学》 2006年第8卷第9期页码 42-48

摘要：

为了解决模型修正过程中修正目标众多和测量信息有限的矛盾，提出了多重子步模型修正方法(MSMUM)，先进行误差定位，再实现参数修正，把复杂的模型修正过程分步实现。利用子结构技术，使结构模型在整体上仅包含有限数目的子结构，对各子结构所对应的超级单元的刚度参与系数进行识别来实现误差定位，通过建立测量信息与待修正参数间的代数方程来修正误差定位后子结构内部构件的参数。数值算例表明，建立的模型修正方法有效可靠。利用建立的多重子步模型修正方法对润扬大桥南汊悬索桥桥塔模型进行了修正。

关键词：模型修正子结构误差定位参数修正润扬桥

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Study on a green synthetic method of TRPPMCl (M= Fe, Mn, Co)

Jing FAN, Yuanbin SHE, Aixin WANG

《化学科学与工程前沿（英文）》 2009年第3卷第2期页码 222-223 doi: 10.1007/s11705-009-0059-y

摘要： A green synthetic method using mixted solvents of di-methyl formamide(DMF) and acids instead of single DMF to synthesize metalloporphyrins(TRPPMCl) from TRPPH and MCl metallization was proposed in this paper. A series of TRPPMCl (M= Fe, Mn, Co) were synthesized through this innovative synthetic method, and these complexes were characterized with various spectroscopic techniques, including IR and UV-Vis spectroscopy. The results showed that the metallization time was markedly reduced from about 10 h to 0.5 h in contrast with the conventional synthetic methods.

关键词： TRPPMCl metalloporphyrins green synthesis acid

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New decentralized control technique based on substructure and LQG approaches

Ying LEI, Ying LIN,

《机械工程前沿（英文）》 2009年第4卷第4期页码 386-392 doi: 10.1007/s11465-009-0041-x

摘要： Decentralized control is more suitable for structural control of large-scale structures. In this paper, a new decentralized control technique is proposed based on the linear quadratic Gaussian (LQG) and substructure approaches. A large-scale structure is divided into a set of smaller substructures. Each substructure is controlled by its own local controller with interaction forces at substructural interfaces, which are considered as “unknown external inputs” to the substructure concerned. An algorithm of recursive least squares estimation for the unknown excitation is proposed. A numerical example of the decentralized control of a tall building is studied to illustrate the new proposed algorithm. Simulation results show that the proposed decentralized control provides satisfactory control performance when comparing with the conventional centralized LQG control algorithm and is viable for the future structural control of large-scale structures.

关键词： substructures decentralized control linear quadratic Gaussian (LQG) Kalman filter unknown input least-squares estimation

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Genome reprogramming for synthetic biology

Kylie Standage-Beier,Xiao Wang

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 37-45 doi: 10.1007/s11705-017-1618-2

摘要： The ability to go from a digitized DNA sequence to a predictable biological function is central to synthetic biology. Genome engineering tools facilitate rewriting and implementation of engineered DNA sequences. Recent development of new programmable tools to reengineer genomes has spurred myriad advances in synthetic biology. Tools such as clustered regularly interspace short palindromic repeats enable RNA-guided rational redesign of organisms and implementation of synthetic gene systems. New directed evolution methods generate organisms with radically restructured genomes. These restructured organisms have useful new phenotypes for biotechnology, such as bacteriophage resistance and increased genetic stability. Advanced DNA synthesis and assembly methods have also enabled the construction of fully synthetic organisms, such as J. Craig Venter Institute (JCVI)-syn 3.0. Here we summarize the recent advances in programmable genome engineering tools.

关键词： CRISPR genome engineering synthetic biology rational design

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Research progress on synthesis of zeolites from coal fly ash and environmental applications

《环境科学与工程前沿（英文）》 2023年第17卷第12期 doi: 10.1007/s11783-023-1749-2

摘要：

● Up-to-date information on the preparation of zeolite from CFA were summarized.

关键词： Coal fly ash Zeolite Synthetic method Environmental application Industrialization

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Renewable synthetic fuel: turning carbon dioxide back into fuel

《能源前沿（英文）》 2022年第16卷第2期页码 145-149 doi: 10.1007/s11708-022-0828-6

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Fire resistance evaluation through synthetic fire tests and generative adversarial networks

《结构与土木工程前沿（英文）》 2024年第18卷第4期页码 587-614 doi: 10.1007/s11709-024-1052-8

摘要： This paper introduces a machine learning approach to address the challenge of limited data resulting from costly and time-consuming fire experiments by enlarging small fire test data sets and predicting the fire resistance of reinforced concrete columns. Our approach begins by creating deep learning models, namely generative adversarial networks and variational autoencoders, to learn the spatial distribution of real fire tests. We then use these models to generate synthetic tabular samples that closely resemble realistic fire resistance values for reinforced concrete columns. The generated data are employed to train state-of-the-art machine learning techniques, including Extreme Gradient Boost, Light Gradient Boosting Machine, Categorical Boosting Algorithm, Support Vector Regression, Random Forest, Decision Tree, Multiple Linear Regression, Polynomial Regression, Support Vector Machine, Kernel Support Vector Machine, Naive Bayes, and K-Nearest Neighbors, which can predict the fire resistance of the columns through regression and classification. Machine learning analyses achieved highly accurate predictions of fire resistance values, outperforming traditional models that relied solely on limited experimental data. Our study highlights the potential for using machine learning and deep learning analyses to revolutionize the field of structural engineering by improving the accuracy and efficiency of fire resistance evaluations while reducing the reliance on costly and time-consuming experiments.

关键词： deep learning fire resistance generative adversarial networks machine learning

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Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE

《化学科学与工程前沿（英文）》 doi: 10.1007/s11705-024-2458-5

摘要： Genomic rearrangements play a crucial role in shaping biological phenotypic diversity and driving species evolution. Synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE) has been applied to explore large-scale genomic rearrangements, yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym sites. Here, we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes, ranging from 1 to 300 kb. Furthermore, it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of zeaxanthin. Collectively, our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关键词： genomic rearrangements synthetic yeast genome SCRaMbLE inter-chromosomal insertions synthetic biology

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Targeting “undruggable” c-Myc protein by synthetic lethality

Chen Wang, Hui Fang, Jiawei Zhang, Ying Gu

《医学前沿（英文）》 2021年第15卷第4期页码 541-550 doi: 10.1007/s11684-020-0780-y

摘要： Synthetic lethal screening, which exploits the combination of mutations that result in cell death, is a promising method for identifying novel drug targets. This method provides a new avenue for targeting “undruggable” proteins, such as c-Myc. Here, we revisit current methods used to target c-Myc and discuss the important functional nodes related to c-Myc in non-oncogene addicted network, whose inhibition may cause a catastrophe for tumor cell destiny but not for normal cells. We further discuss strategies to identify these functional nodes in the context of synthetic lethality. We review the progress and shortcomings of this research field and look forward to opportunities offered by synthetic lethal screening to treat tumors potently.

关键词： synthetic lethality undruggable transcription factor c-Myc

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SCRaMbLE drive application of synthetic yeast genome

Jin Jin, Yuan Ma, Duo Liu

《化学科学与工程前沿（英文）》 2018年第12卷第4期页码 832-834 doi: 10.1007/s11705-018-1749-0

摘要：

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Water-soluble BODIPY and aza-BODIPY dyes: synthetic progress and applications

Gang FAN,Le YANG,Zhijian CHEN

《化学科学与工程前沿（英文）》 2014年第8卷第4期页码 405-417 doi: 10.1007/s11705-014-1445-7

摘要： In recent years, boron-dipyrromethene (BODIPY) and boron-azadipyrromethene (aza-BODIPY) dyes have attracted considerable multidisciplinary attention due to their diverse applications. By introducing various hydrophilic groups, such as quaternary ammonium, sulfonate or oligo-ethyleneglycol moieties into the BODIPY core, the solubilities of these dyes in aqueous solution can be greatly improved while maintaining their high fluorescence quantum yields. Accordingly, applying these fluorescent dyes in aqueous systems to areas such as chemosensors, biomacromolecule labeling, bio-imaging and photodynamic therapy has been achieved. In this article, the recent progress on the synthesis, optical properties and application of water-soluble BODIPY dyes and aza-BODIPY dyes is reviewed.

关键词： boron-dipyrromethene boron-azadipyrromethene synthetic progress applications

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Epigenetic silencing of BEND4, a novel DNA damage repair gene, is a synthetic lethal marker for ATM inhibitor

《医学前沿（英文）》 doi: 10.1007/s11684-023-1053-3

摘要： Synthetic lethality is a novel model for cancer therapy. To understand the function and mechanism of BEN domain-containing protein 4 (BEND4) in pancreatic cancer, eight cell lines and a total of 492 cases of pancreatic neoplasia samples were included in this study. Methylation-specific polymerase chain reaction, CRISPR/Cas9, immunoprecipitation assay, comet assay, and xenograft mouse model were used. BEND4 is a new member of the BEN domain family. The expression of BEND4 is regulated by promoter region methylation. It is methylated in 58.1% (176/303) of pancreatic ductal adenocarcinoma (PDAC), 33.3% (14/42) of intraductal papillary mucinous neoplasm, 31.0% (13/42) of pancreatic neuroendocrine tumor, 14.3% (3/21) of mucinous cystic neoplasm, 4.3% (2/47) of solid pseudopapillary neoplasm, and 2.7% (1/37) of serous cystic neoplasm. BEND4 methylation is significantly associated with late-onset PDAC (> 50 years, P < 0.01) and tumor differentiation (P < 0.0001), and methylation of BEND4 is an independent poor prognostic marker (P < 0.01) in PDAC. Furthermore, BEND4 plays tumor-suppressive roles in vitro and in vivo. Mechanistically, BEND4 involves non-homologous end joining signaling by interacting with Ku80 and promotes DNA damage repair. Loss of BEND4 increased the sensitivity of PDAC cells to ATM inhibitor. Collectively, the present study revealed an uncharacterized tumor suppressor BEND4 and indicated that methylation of BEND4 may serve as a potential synthetic lethal marker for ATM inhibitor in PDAC treatment.

关键词： BEND4 DNA methylation synthetic lethality NHEJ pathway

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Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 58-65 doi: 10.1007/s11705-017-1610-x

摘要： The advent of synthetic biology has ushered in new applications of cell-free transcription-translation systems. These cell-free systems are reconstituted using cellular proteins, and are amenable to modular control of their composition. Here, we discuss the historical advancement of cell-free systems, as well as their new applications in the rapid design of synthetic genetic circuits and components, directed evolution of biomolecules, diagnosis of infectious diseases, and synthesis of vaccines. Finally, we present our vision on the future direction of cell-free synthetic biology.

关键词： cell-free system application

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Hollow-fiber gas penetration electrodes efficiently produce renewable synthetic fuels

《能源前沿（英文）》 2022年第16卷第5期页码 700-705 doi: 10.1007/s11708-022-0842-8