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期刊论文 4

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2023 1

2021 1

2018 1

2005 1

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生物转化 2

两相分配 1

他汀类药物 1

化学-酶合成 1

微生物发酵 1

浊点系统 1

生物制造 1

生物反应器 1

非离子表面活性剂 1

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Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

《化学科学与工程前沿（英文）》 2021年第15卷第3期页码 615-629 doi: 10.1007/s11705-020-1989-7

摘要： A cholesterol oxidase (COD) was hybridized with Ca , Zn , Al , Fe and Mn . After precipitation with PO at 4 °C for 72 h, the resulting pellets were freeze-dried. In scanning electron microscopy assays, the metal-COD complexes revealed flower-like or granular structures after hybridization. Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials. X-ray diffraction analysis indicated that COD was encapsulated in CaHPO ·2H O-, Zn (PO ) ·4H O-, AlPO -, FeP - and Mn (PO ) ·3H O-based nanostructures, respectively. Differential scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5 °C to 167.02 °C, 167.02 °C, 137.70 °C, 172.85 °C and 160.99 °C, respectively. Using steroid derivatives as substrates, this enzyme could convert cholesterol, pregnenolone, dehydroepiandrosterone, ergosterol, -sitosterol and stigmasterol to related single products. Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme. In addition, substrate selectivity could be affected by various metal materials. Briefly, using Ca , Zn , Al , Fe and Mn as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures, and could help to promote the stability and tolerant properties of the enzyme, while also enhancing its catalytic characteristics.

关键词： cholesterol oxidase metal-enzyme hybridization nanostructures sterol derivatives bioconversion

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两相分配生物反应器——浊点系统在生物转化中的应用

王志龙

《中国工程科学》 2005年第7卷第5期页码 73-78

摘要：

利用两相分配生物反应器可以控制底物由非水相向水相释放，增加底物的溶解度和解除底物对微生物的抑制，保护产物降解，降低下游分离费用；论述了两相分配生物反应器的基本原理和发展概况，并以胆固醇边链切除生物转化为例，介绍了新开发的浊点系统两相分配生物反应器的巨大潜力。

关键词：两相分配生物转化非离子表面活性剂浊点系统生物反应器

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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

《化学科学与工程前沿（英文）》 2018年第12卷第4期页码 772-779 doi: 10.1007/s11705-018-1731-x

摘要： We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet- T7- T7- was constructed and transformed to BL21(DE3) to obtain the whole cells of BL21- capable of bioconversion D-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L yeast extract, 5 g·L sucrose, 3g·L KH PO , 5 g·L NaCl, 3g·L NH Cl, 0.25 g·L MgSO ?7H O and 1 mL·L the mixture of trace elements. With the optimized whole cells of BL21- , 60 g·L of xylose was converted to 28g·L BT with a molar yield of 66 %, which is higher than those reported in the biotechnological system.

关键词： D-1 2 4-butanetriol whole-cell bioconversion carbon source nitrogen sources metal ions culture conditions

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从他汀类药物的发现、发展和创新看生物制造的重要性 Review

汤晓玲, 俞佳薇, 耿宇恒, 王嘉瑞, 郑仁朝, 郑裕国

《工程（英文）》 2023年第24卷第5期页码 138-150 doi: 10.1016/j.eng.2022.04.030

摘要：

先进工业的快速发展正在引发化学品生产模式的变革。生物制造是在细胞及其组分介导下进行物质加工与合成的绿色先进生产方式，具有原子经济性高、副反应少、过程需求适应性强及环境友好等优势，可以革新加工原料供给、强化和优化生产过程、提升产品质量。生物制造的重要性在他汀类药物的发现、发
展和创新过程中得到了有力体现。他汀类药物作为治疗和预防心脑血管病的基石，开创了心脑血管病治疗的新纪元，在人类医药发展史上具有重要意义。从微生物发酵生产第一代他汀类药物、生物合成生产第二代他汀类药物到化学-酶法合成第三代他汀类药物，可以看到生物制造技术对他汀类药物的巨大推动力，对变革生产路线与生产工艺、降低碳排放、提高生产效率具有重要意义。随着前沿生物制造技术的发展及与多学科的交叉融合，生物制造技术将形成他汀类药物新的生产力，进一步推动其健康可持续发展。

关键词：生物制造他汀类药物微生物发酵生物转化化学-酶合成