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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

《农业科学与工程前沿（英文）》 2017年第4卷第1期页码 48-58 doi: 10.15302/J-FASE-2016114

摘要： Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

关键词： Puccinia striiformis f. sp. tritici selfing heterozygosity virulence inheritance

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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿（英文）》 2017年第4卷第1期页码 121-122 doi: 10.15302/J-FASE-2017142

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不同类型意大利蜜蜂MDH Ⅱ同工酶的研究

陈盛禄,鲍秀良,苏松坤,刘艳荷

《中国工程科学》 2000年第2卷第1期页码 57-61

摘要：

运用聚丙烯酰胺凝胶等电聚焦技术（IEF-PAGE），对有代表性的王浆蜂蜜双高产的"浙农大1号"意蜂品种（浙意，Ea）和中国本地意蜂（本意，Eb）、美国意蜂（美意，Em）、澳大利亚意蜂（澳意Eo）、意大利意蜂（原意，Ee）等5个意大利蜂（Apis mellifera Ligustica）品种试飞前的幼龄工蜂进行了苹果酸脱氢酶（MDH）同工酶的基因型、基因型频率、基因频率、杂合度和纯合度的研究。由a、b、c 3个等位基因编码的MDH图谱第二区带应有的a/a、a/b、a/c、b/b、b/c和c/c 6种基因型，在Ea、Eb、Ee 3种意蜂中全部出现；在Eo中出现a/b，a/c，b/c，c/c 4种，在Em中出现a/c，b/c，c/c 3种。联列表独立性检验结果，5个意蜂品种间的基因型频率、基因频率、杂合度、纯合度存在显著差异。

关键词：意蜂品种苹果酸脱氢酶基因型基因频率杂合度