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2008, Volume 2, Issue 4

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Frontiers of Medicine >> 2008, Volume 2, Issue 4 doi: 10.1007/s11684-008-0073-3

Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

1.Department of Occupational and Environmental Health, the Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology；Institute for Chemical Carcinogenesis, Guangzhou Medical College; 2.Institute for Chemical Carcinogenesis, Guangzhou Medical College; 3.Department of Occupational and Environmental Health, the Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology;

Available online: 2008-12-05
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Abstract

Anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (anti-BPDE) is a metabolite of benzo[a]pyrene (B[a]P) and acts as a potent mutagen in mammalian systems. However, molecular mechanisms related to anti-BPDE-induced carcinogenesis are poorly understood. Here, we investigated the expression of proto-oncogene c- in human bronchial epithelial cells (16HBE-T) transformed by exposure to anti-BPDE. The levels of mRNA and protein of c-Myc were examined in the 16HBE-T and vehicle-treated control cells (16HBE-N) by using different methods respectively, including reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR (Q-PCR), western blot and immunocytochemical methods. The level of c- mRNA appeared to be significantly increased in 16HBE-T, as compared with those of the 16HBE-N. Likewise, the expression of c-Myc protein was significantly enhanced as compared with those of the control cells. Moreover, the localization of c-Myc protein shows mainly nuclear staining in 16HBE-T. In conclusion, the abnormal expression of c-Myc was present in anti-BPDE malignantly transformed 16HBE cells, which may be involved in the carcinogenesis molecular mechanism of anti-BPDE.

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