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denaturing gradient gel electrophoresis (DGGE) 2

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Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 87-92 doi: 10.1007/s11684-007-0017-3

Abstract: Reverse transcriptase-polymerase chain reaction was used to detect mRNA expressions of adipose leptin

Keywords: significant difference     intestinal I/R     transcriptase-polymerase     metabolic     central secretory    

Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

FU Juan, JIANG Yiguo, CHEN Xuemin

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 380-385 doi: 10.1007/s11684-008-0073-3

Abstract: vehicle-treated control cells (16HBE-N) by using different methods respectively, including reverse transcriptase-polymerase

Keywords: transcriptase-polymerase     vehicle-treated     understood     molecular mechanism     different    

Effects of 3-aminobenzamide on poly (ADP-ribose) polymerase expression, apoptosis and cell cycle progression

DU Xiang, ZHAO Hongguang, GUO Wei, GONG Shouliang, WANG Wen

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 204-206 doi: 10.1007/s11684-008-0039-5

Abstract: study the changes of apoptosis and cell cycle progression in HeLa cells after the poly (ADP-ribose) polymerase

Keywords: control     irradiation     apoptotic     progression     3-AB    

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 21-27 doi: 10.1007/s11783-011-0303-9

Abstract: In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of

Keywords: polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 166-174 doi: 10.1007/s11684-014-0334-2

Abstract: In late 2013 the first nucleotide polymerase inhibitor regimen with RBV alone for genotypes 2/3 and in

Keywords: direct-acting antiviral (DAA)     nucleotide polymerase inhibitors     protease inhibitors    

Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Strategic Study of CAE 2007, Volume 9, Issue 7,   Pages 76-81

Abstract:

In this paper a new style of polymerase chain reaction(PCR)equipment

Keywords: microreactors     measurement     control     polymerase chain reaction    

Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 269-275 doi: 10.1007/s11684-008-0051-9

Abstract: fingerprint maps, subsequently, using selected RAPD primer could give out a group of amplification polymerase

Keywords: amplification polymerase     CTAB     sequence     cetyltrimethylammonium     RAPD    

Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 197-203 doi: 10.1007/s11684-009-0036-3

Abstract: The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription polymerase chain reaction

Keywords: bladder     phosphatase of regenerating liver 1     phosphatase of regenerating liver 3     reverse transcription polymerase    

Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 40-47 doi: 10.1007/s11783-011-0304-8

Abstract: community shift along with environmental variations using denaturing gradient gel electrophoresis of polymerase

Keywords: sewage treatment plants     polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE    

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords: quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 2,   Pages 324-333 doi: 10.1007/s11783-014-0627-3

Abstract: The microbial community structure of the granules was also analyzed by polymerase chain reaction-denaturing

Keywords: sludge     completely mixed activated sludge system (CMAS)     continuous flow     shear force     filamentous bacteria     polymerase    

Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 28-39 doi: 10.1007/s11783-011-0302-x

Abstract: Real-time quantitative polymerase chain reaction (qPCR) has gained popularity as a technique to detect

Keywords: absolute quantification     design guideline     primer     probe     real-time quantitative polymerase chain reaction    

A DNA Computing Model for the Graph Vertex Coloring Problem Based on a Probe Graph Article

Jin Xu, Xiaoli Qiang, Kai Zhang, Cheng Zhang, Jing Yang

Engineering 2018, Volume 4, Issue 1,   Pages 61-77 doi: 10.1016/j.eng.2018.02.011

Abstract: vertices in subgraphs; and②the bio-operation times are reduced considerably by a designed parallel polymerase

Keywords: DNA computing     Graph vertex coloring problem     Polymerase chain reaction    

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 204-207 doi: 10.1007/s11684-010-0043-4

Abstract: number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase

Keywords: Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0846-x

Abstract: various processes of municipal WWTPs by employing two molecular-based methods:most probable number-polymerase

Keywords: DNA microarray analysis     Nitrogen cycling functional genes     Most probable number-polymerase chain reaction    

Title Author Date Type Operation

Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie

Journal Article

Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

FU Juan, JIANG Yiguo, CHEN Xuemin

Journal Article

Effects of 3-aminobenzamide on poly (ADP-ribose) polymerase expression, apoptosis and cell cycle progression

DU Xiang, ZHAO Hongguang, GUO Wei, GONG Shouliang, WANG Wen

Journal Article

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Journal Article

Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Journal Article

Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Journal Article

Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Journal Article

Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Journal Article

Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Journal Article

Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Journal Article

A DNA Computing Model for the Graph Vertex Coloring Problem Based on a Probe Graph

Jin Xu, Xiaoli Qiang, Kai Zhang, Cheng Zhang, Jing Yang

Journal Article

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Journal Article

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

Journal Article