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2008 1

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amplification polymerase 1

cetyltrimethylammonium 1

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Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 269-275 doi: 10.1007/s11684-008-0051-9

Abstract: The aim of this paper is to develop an applicable Random Amplified Polymorphic DNAs (RAPD) method forgenotyping strain, and discuss the possibility of using the RAPD method to trace strain transmissionDifferent RAPD primers sequence was used for amplification and their differentiating capabilities forApplicable RAPD primer was selected for genotyping and then applied into transmission detection.Based on these findings, the best extracting method and suitable RAPD primer were chosen.

Keywords： amplification polymerase     CTAB     sequence     cetyltrimethylammonium     RAPD    

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Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Journal Article