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Measurement and Control Technology for the Array of Low Cost PCR Microreactors
Li Xianming ,Zhang Yulin ,Li Jiantian
Strategic Study of CAE 2007, Volume 9, Issue 7, Pages 76-81
In this paper a new style of polymerase chain reaction(PCR)equipment is studied and the goal of the study is to reduce the cost of micro-reactor PCR chips, improve its reliability and make a hundred of chips working together in the same time. There are no micro-sensors and micro-heaters in the chips. It is the concrete way to reduce the cost of PCR chips and improve the reliability of the chips. So the focus of the study transfers from the design, simulation and processing to the measurement and control of the chips. The measurement and control scheme for the low-cost PCR chips is designed. By selecting several chips as model chips and combining unitized macroscopic control and distributed microcosmic control, the system is able to do temperature cycling control to the chip array which is made of many chips at the same time. The loading effect applied to the temperature field by the temperature sensor and the compensation method for the loading effect are studied. The main idea of this method is to compare the measurement result gained from the temperature field in which two sensors are set with the measurement result gained from the same temperature field but in which one sensor is set, and the difference is the loading effect that one sensor applies to the temperature field. The temperature measurement errors to the liquid internal the model chips are analyzed and synthesized in considerable detail and an intelligent prediction compensation method for dynamic measurement errors is obtained. The uncertainty of indirect to the temperature of PCR liquid in low-cost PCR chips is also assessed. Cascade control strategy is applied to further improving the performance of control system. Its performance in temperature cycling meets the requirements of PCR.
Keywords： microreactors measurement control polymerase chain reaction
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