2016, Volume 10, Issue 3
Frontiers of Medicine >> 2016, Volume 10, Issue 3 doi: 10.1007/s11684-016-0459-6
Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium
1. Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200031, China.
2. Central Laboratory, Affiliated Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
3. Key Laboratory of Hearing Medicine of the National Health and Family Planning Commission, Shanghai 200031, China.
4. MOE Key Laboratory of Developmental Genes and Human Disease, State Key Laboratory of Bioelectronics, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
5. Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
6. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
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Abstract
The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine N-methyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.
Keywords
polycomb repressive complex ; Ezh2 ; expression ; inner ear ; cochlea ; development
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