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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 10, doi: 10.1007/s11783-022-1541-8

Abstract:

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.

Keywords： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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Determination of 27 pharmaceuticals and personal care products (PPCPs) in water: The benefit of isotope dilution

Xueqi Fan, Jie Gao, Wenchao Li, Jun Huang, Gang Yu

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1187-3

Abstract: isotope dilution method using liquid chromatography coupled to tandem triple quadruple mass spectrometer (LC-MS/MS).

Keywords： personal care products (PPCPs)     Isotopically labeled standard (ILSs)     Water     Solid-phase extraction (SPE)     LC-MS/MS    

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LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 436-442 doi: 10.1007/s11705-012-1222-4

Abstract: In this paper, chromatography/tandem mass spectrometry (LC/MS/MS) combined with radioisotope method wasAnd samples were precipitated with methanol and quantified with LC/MS/MS.

Keywords： catalpol     LC/MS/MS     OCT2    

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Simultaneous quantification of several classes of antibiotics in water, sediments, and fish muscles by liquid chromatography–tandem mass spectrometry

WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 3,   Pages 357-371 doi: 10.1007/s11783-013-0580-6

Abstract: Liquid chromatography–tandem mass spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM) detection

Keywords： antibiotics     liquid chromatography–tandem mass spectrometry (LC-MS/MS)     water     sediment     fish muscle    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 216-219 doi: 10.1007/s11684-010-0037-2

Abstract: To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

Keywords： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 277-283 doi: 10.1007/s11684-009-0047-0

Abstract: Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10 and 4.096×10, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0―4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125g/L; 12 of 15 IEM HBsAg species (P/N≥2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450nm (), one had an intermediate value and nine had a high value, which was 7.55%(mean), 59.4% and 92.1%―109.4% of the wild-type value, respectively. The two species with low value and the three negative species mutated at the bases 120―124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% 14.89% and 6.21%, respectively; <0.01, <0.05, respectively).

Keywords： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbent assay    

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Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 185-191 doi: 10.1007/s11684-010-0040-7

Abstract: The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 1255 cases of people living with HIV/AIDS. Among the newly discovered 145 cases of TB/HIV co-infection, AIDS institutions found 86.9% of these co-infections and 14.1% came from TB institutes. In different registration categories, there were 31 (21.4%) cases of smear positive TB, 104 (71.7%) cases of smear negative TB, and extrapulmonary cases accounted for 10 (6.9%). Gender, occupation, marital status, ethnic group, education, HIV transmission route, CD4 level, and type of TB susceptible symptoms had significant effects on whether HIV/AIDS patients were co-infected with TB disease (<0.01). Sex, age, ethnic group, occupation, and type of tuberculosis had no significant influence on whether TB patients had HIV infection. AIDS institutions could find more TB/HIV co-infection patients in comparison with TB institutions, particularly in patients with smear negative pulmonary tuberculosis and extrapulmonary tuberculosis. Therefore, a cooperation mechanism should be built up between TB and HIV control systems, and routine TB checks should be conducted for HIV/AIDS patients. In areas where sex or drug use is the major transmission mode of HIV infection, antiretroviral therapy should be expanded, treatment adherence should be improved and patients’ immunity level should be increased in order to decrease TB incidence. Cough and expectoration of more than two weeks, bloody sputum, and recurrent fevers could be revealing symptoms in TB screening of HIV/AIDS patients.

Keywords： tuberculosis/acquired immunodeficiency syndrome     dual infection     prevention and control     case detection     impact factors    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 204-207 doi: 10.1007/s11684-010-0043-4

Abstract: Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

Keywords： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Strategic Study of CAE 2008, Volume 10, Issue 2,   Pages 14-25

Abstract: precursors-the gestation process and the signal of impending earthquake of the Indian Ocean Earthquake of Msthat there are great differences for the stress in crust between before and after the eart hquake of Ms8-7 and that of Ms 8-5.tsunami is analyzed.The sign of underground material migration is also found in the gestation process of Ms

Keywords： Ms 8-7 and Ms 8-5 earthquake in Indian Ocean     earthquqke precursor     signal of impending earthquake     tsunami    

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The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1529-4

Abstract:

• The resistance of phage PhiX174 to nZVI was much stronger than that of MS2

Keywords： Nanoscale zero-valent iron (nZVI)     MS2     PhiΧ174     Resistance     Inactivation     Pathogenic microorganisms    

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Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 503-506 doi: 10.1007/s11684-009-0076-8

Abstract: We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma.

Keywords： teratoma     parotid gland    

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and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 4,   Pages 547-552 doi: 10.1007/s11783-013-0620-2

Abstract: In this study, inactivation of and bacteriophage MS2 by UV, ozone, UV/ozone coexposure, and sequentialThe synergistic effect on MS2 inactivation was less significant after the UV/ozone coexposure, and more

Keywords： bacteria inactivation     photoreactivation     water disinfection     UV     ozone    

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Detection of maternal serum thromboxane B2 in different durations of pregnancy and prediction of preeclampsia

Geqing XIA MD, Zehua WANG MD, Li ZOU MD, Chaoying WU MS, Yu HU MD,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 235-240 doi: 10.1007/s11684-010-0002-0

Abstract: This study was aimed to detect maternal serum levels of thromboxane B2 (TXB2) in different durations of pregnancy and explore the predictive value of TXB2 for preeclampsia. By employing a prospective and double-blind study method, 180 pregnant women with previously normal blood pressures during their progestation were included in the study. Peripheral venous blood samples were obtained during 10–14 (period I), 20–24 (period II) and 30–34 (period III) weeks of gestation. Maternal serum levels of TXB2 were measured by enzyme-linked immunoassay. The pathogenetic condition and pregnancy outcomes of these cases were observed. (1) Among the 180 previously normotensive women, ten developed preeclampsia (case group; four severe and six mild preeclampsia). One hundred and seventy remained normal till the end of pregnancy (control group). (2) With development of pregnancy, the levels of maternal serum TXB2 in the 180 cases gradually ascended as seen through the Wilcoxon Signed Ranks Test. There was a statistical significance in maternal TXB2 levels between two different gestational stages (<0.01). (3) The levels of maternal serum TXB2 were slightly higher in the case group than in the control group during period I of gestation, but the difference was not statistically significant (>0.05); the levels of TXB2 were significantly higher in the case group than in the control group during periods II and III of gestation (both <0.05). (3) The best cutoff points of maternal TXB2 were 3750 and 4400ng/mL during periods II and III of gestation by receiver operator characteristic (ROC) curve; and the sensitivity, specificity, positive predictive value, negative predictive value and odds radio of TXB2 in predicting preeclampsia were 80%, 69.5%, 13.3%, 98.33%, 9.11 and 90%, 68.82%, 13.84%, 99.13, 19.86, respectively. Higher levels of maternal serum TXB2 were detected a long time before clinical symptoms appeared. The maternal serum TXB2 after 20 weeks of gestation had predictive value, and the index after 30 weeks was superior to that prior to 30 weeks .

Keywords： thromboxane B2     preeclampsia    

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Sensory innervation of the anterior eye segment in rats: a retrograde tracing study

Haixia LIU MD , Zhiwang LI MD , Min YANG MD , Xiang TIAN MS , Chaoying LI PhD , Lei PEI PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 352-356 doi: 10.1007/s11684-009-0065-y

Abstract: This study aimed to investigate the sensory innervation of the anterior eye segment in rats by retrograde tracing with 1,1-dioleyl-3,3,3,3-tetrameth-ylindocarbocyanine, 4-chlorobenesulfonate (FAST Dil) injected into the anterior chamber. In our study, the sensory innervation of distinct elements of the anterior segment of the rat’s eye, i.e. the cornea, ciliary body, iris, and trabecular meshwork, were studied by retrograde tracing using FAST Dil as a tracer. FAST Dil was injected into the anterior chambers of the rat’s eyes. The animals were sacrificed at different time points, i.e., 2, 3, 4, and 5 days after the injection. FAST Dil localization in trigeminal ganglions was studied with a fluorescent microscope. Two days after FAST Dil injection into the anterior chambers, the cornea, the ciliary body, the iris, and the trabecular meshwork were heavily labeled. Neurons in the ipsilateral trigeminal ganglion were also consistently labeled. The number of labeled cells increased over time until 4 days after FAST Dil injection. FAST Dil-labeled neurons could be divided into two parts. Most of the Dil-labeled neurons were concentrated in a sharp, longitudinal, spindle-like stripe, located in the dorso-medial side of the trigeminal ganglion, approximately two thirds of the dorsal portion. The other part of Dil-labeled neurons scattered laterally to the stripe, but just in one third of the dorsal region. Thus, with our preliminary results, we conclude that the primary afferent sensory neurons innervating the rat’s anterior eye segments aggregate in the dorso-medial part of the ipsilateral trigeminal ganglion. It is feasible to identify them using retrograde tracing with FAST Dil anterior chamber injection.

Keywords： trigeminal ganglion     retrograde tracing    

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Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Dai-Hai YU PhD, Jian-Feng HUANG MD, Ji-Chun CHEN MS, Jie CAO MS, Shu-Feng CHEN PhD, Dong-Feng GU MD,HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K. WHELTON MD, MSc9,

Frontiers of Medicine 2010, Volume 4, Issue 1,   Pages 59-66 doi: 10.1007/s11684-010-0015-8

Abstract: Dietary potassium-supplementation has been associated with a decreased risk of hypertension and other cardiovascular outcomes. However, blood pressure (BP) responses to potassium supplementation vary among individuals. This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all <0.0001) that remained significant after adjustment for multiple comparisons. In participants that were heterozygous or homozygous for the G allele of this marker, SBP, DBP, and MAP response to potassium-supplementation were −3.52 (−3.82, −3.21), −1.41 (−1.66, −1.15) and −2.12 (−2.37, −1.87), respectively, as compared to the corresponding responses of 1.99 (0.25, 3.73), −0.65 (−0.10, −0.21), and −0.23 (−0.37, 0.83), respectively, for those who were homozygous for A allele. In addition, participants with at least one copy of the G allele of rs12503220 of the ADD1 gene had significantly increased DBP and MAP response to potassium-supplementation ( = 0.0041 and 0.01, respectively), which was also significant after correction for multiple testing. DBP and MAP responses to potassium-supplementation were −1.36 (−1.63, −1.10) and −2.07 (−2.32, −1.82) for those with at least G allele compared to corresponding responses of 0.86 (−0.68, 2.40) and −0.45 (−1.74, 0.84) for those who were homozygous for A allele. In summary, our study identified novel associations between genetic variants of the ADD1 gene and BP response to potassium-supplementation, which could have important clinical and public health implications. Future studies aimed at replicating these novel findings are warranted.

Keywords： blood pressure     genetics     polymorphism     die-tary potassium     potassium sensitivity     adducin 1 alpha (ADD1)     guanine nucleotide binding protein beta polypeptide 3 (GNB3)    

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Title Author Date Type Operation

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Journal Article

Determination of 27 pharmaceuticals and personal care products (PPCPs) in water: The benefit of isotope dilution

Xueqi Fan, Jie Gao, Wenchao Li, Jun Huang, Gang Yu

Journal Article

LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Journal Article

Simultaneous quantification of several classes of antibiotics in water, sediments, and fish muscles by liquid chromatography–tandem mass spectrometry

WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong

Journal Article

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Journal Article

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Journal Article

Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Journal Article

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Journal Article

The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Journal Article

The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Journal Article

Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Journal Article

and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

Journal Article

Detection of maternal serum thromboxane B2 in different durations of pregnancy and prediction of preeclampsia

Geqing XIA MD, Zehua WANG MD, Li ZOU MD, Chaoying WU MS, Yu HU MD,

Journal Article

Sensory innervation of the anterior eye segment in rats: a retrograde tracing study

Haixia LIU MD , Zhiwang LI MD , Min YANG MD , Xiang TIAN MS , Chaoying LI PhD , Lei PEI PhD ,

Journal Article

Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Dai-Hai YU PhD, Jian-Feng HUANG MD, Ji-Chun CHEN MS, Jie CAO MS, Shu-Feng CHEN PhD, Dong-Feng GU MD,HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K. WHELTON MD, MSc9,

Journal Article