In the article, the application of biological technique in the scientific research of sheep husbandry is introduced. It involves frozen semen, embryo transfer, embryo freezing preservation, embryo splitting, in vitro fertilization, nuclear transfer and cloned embryo, gene transfer and so on. The application of biological technique not only improves the level of sheep breeding and production, but also brings about obvious economical results. Meanwhile, new ways and channels are opened up for scientific research and production in sheep husbandry by the development and maturation of biological technique.