By means of lab test, enlarged test and industrial test of biopurifying the waste gas containing toluene (VOC) in low concentration, the biopurifying technology of organic waste gas was researched systematically. In a rubber-regeneration factory, the industrial test on purifying the low concentration organic waste gas (containing sulfur) from rubber-regeneration process by using biochemical method was carried through. For the waste gas containing toluene in a range of 300 〜1400 mg/m³, the industrial test device showed a good purification capacity. The result of 100 d test running with the device indicated that the purification efficiency of toluene in the rubber-regeneration waste gas could be kept at 90 % for a long time and the treated waste gas could be discharged in the situation of satisfying the National Emission Standard. The biopurifying technology of waste gas displayed its good advancement of technology and rationality of economy.