A conception of “un-stressing concentration” anti-fatigue for ultra-high strength steel 300 M has been investigated. The compress stress field and cell structure with high density dislocation were formed in surface layer of specimens with the surface integrity machine, shot peening, hole extrusion and screw rolling strengthening, by which the crack initiation was changed to the subsurface from surface, crack propagation was thwarted, fatigue limit at the place of stress concentration were returned to a approximate to the innate fatigue limit of 300 M steel and “un-stressing concentration” surface integrity condition was resulted. This conception of “un-stressing concentration” anti-fatigue was described as a comprehensive equi-fatigue strength, which was equal approximately to the innate fatigue limit of 300M steel, encirclement on the constructure surface case.