The paper presents the traffic volume and loading of Yangtze River bridges in Jiangsu Province from 2007 to 2010. The results show that traffic volume is increasing along with the time. The traffic volume and loading of truck of Yangtze River Bridge are analyzed by wheel load spectrum. The paper gives the truck wheel load and typical wheel load ratio in Jiangsu Province. The overloading analysis show that overloading is serious in Yangtze River Bridges in Jiangsu Province.The research results is useful to the steel deck pavement design of Taizhou Bridge.