Objective: to evaluate the incorporation of BPA by glioma cell lines, and to observe its relationship with the temperature and the concentration of BPA. Methods: C6, U251 and rat astrocyte cells were incubated in a culture medium, in which ¹⁰B concentration was 20, 40, 60, 80, 100 μg/mL for 24 h. Boron concentration in the cells was measured induced couple plasma-atomic emission spectroscopy (ICP-AES). C6 cells were pre-incubated for 24 h with different boron concentrations in growth medium. Then the mediums were changed to boron-free ones, and boron content was assessed after 1, 2, 3 h. Results: The content of the ¹⁰B in cells was increasing with the increasing concentration of BPA, and the boron concentration ratios of glioma cells to astrocyte are 2.2. The BPA efflux is slower at the lower temperature. Conclusion: BPA has a selectivity for glioma cells, and the results of the efflux assay confirm the temperature dependence of the BPA transport out of the cells.