The concept of biofabrication was provided by School of Mechanical Engineering, Xi'an Jiaotong University since the 1990s. Using the engineering methods to solve the medical issues, we have made some achievements and development on the implants for bone and cartilage, liver, intestinal tissue as well as brain. This paper emphasized on the lastest research work of the customized artificial prosthesis, bioactive bone and joint, and tissue engineered liver in our laboratory, which was corporated with the experts and sciencists of various areas from medicine, biology as well as materials for more than 20 years. Furthermore, it also explored the problems and prospects for the development and application of biofabrication.