Aiming at the impact of load imposed order of balance weight and ship compartments on the the deformation of pillar and built-in structure parts, the construction order of ship compartments chamber and equipment installation sequence scheme are studied, which Solved the technical problems that the impact of load imposed order of balance weight and ship compartments on the the deformation of nut column, rack ,and vertical direction displacement. According to the simulation results of pillar structure, the impact of the ship chamber and balance weight loading sequence to the nut, rack and vertical guide displacement in the direction across the river, along the river and vertical is analyzed. Reasonable ship lift ship compartments chamber construction procedure and construction schedule arrangement are set by optimizing the construction procedures. The construction procedures can make nut, rack, vertical direction, the structure of the ship compartments and pillar under various operating conditions on the displacement meet the safety requirements. And the procedures based on the construction progress of the construction can meet the requirements of ship lift commissioning. Engineering practice proved that chamber construction procedures of Three Gorges Project lift ship established in this paper are reasonable and scientific which ensure the smooth construction of ship chamber of Three Gorges Project lift ship and guarantee the safe operation of ship lift. these construction procedures can provide a reference for similiar project.