Smart polymeric gating membranes are composed of smart polymers and non-stimuli-responsive substrate membrane. The smart membranes can automatically change the hydrophilicity/hydrophobicity on the surface as well as trans-membrane permeability and selectivity in response to the environmental stimuli， which is attributable to the stimuli-responsive variation of hydrophilicity/hydrophobicity and configuration of the smart polymers in the membrane. Smart polymeric gating membranes are used for anti-fouling filtration， affinity separation， “on/off” control of enzyme reaction and controlled release， etc. The way to prepare such membranes affects the important parameters of smart polymeric gating membranes， such as the stimuli-responsive characteristics， stability and reproducibility. There are three kinds of preparation methods to fabricate the smart polymeric gating membranes according to when the smart polymers are added into the membrane materials. They are the methods introducing smart polymers by the modification of substrate membranes materials after membrane preparation， by the modification of substrate membrane materials before membrane preparation and by blending smart polymers during the membrane preparation. The definitions， subcategory， mechanisms and new advances of these preparation methods are introduced separately， and their advantages and disadvantages are addressed. The method that introduces smart polymers by the modification of substrate membranes materials after membrane preparation is the most widely employed to form the smart polymeric gating membranes. However， the method that blends smart polymers during the membrane preparation has great potentials to the mass production of smart polymeric gating membranes in the future. This paper will provide valuable guidance for the efficient preparation of smart polymeric gating membranes with stable and satisfactory stimuli-responsive characteristics.